Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao最新文献

Objective: To investigate the role of Fluor Protector in the demineralization of bovine enamel after exposure to some beverages.

Methods: Twenty-four bovine teeth were divided into experimental and control groups. The enamel specimens of the experimental group were pretreated with Fluor Protector and then exposed to beverages; the enamel specimens of the control group were exposed to beverages directly. All the enamel specimens were exposed to beverages 10 times a day and 5 minutes each time. After 7 days' exposure to the beverages, all the specimens were observed by scanning electron microscope (SEM).

Results: Different degrees of solubilization of enamel prisms in the control group were demonstrated by SEM. The solubilization of enamel prisms in the experimental group was much more slight than that in the control group.

Conclusion: Fluor Protector can inhibit the demineralization of enamel caused by beverages.

Objective: To find out the status and distribution characteristics of senile cataract, which is one of the common disease affecting the quality of life of the elderly people.

Methods: One thousand and sixteen people aged 60 or more in the urban and rural areas of Chengdu were included by cluster random sampling and investigated by questionnaire method and medical examination.

Results: It was found that the prevalence of senile cataract was 52.3% in urban area and 51.3% in rural area. The prevalence was higher in women than in men, and it increased with age. The prevalence of senile cataract by past diagnosis was lower than that by present medical examination; this difference was found obvious in the rural area.

Conclusion: The prevention and treatment of senile cataract should be strengthened.

Objective: To explore the effect of amrinone and aprotinin on expression of leukocyte adhesion molecule in patients with prosthetic valve replacement during perioperative period.

Methods: Thirty-two patients undergoing valve replacement were randomized to control group, the first aprotinin group, the second aprotinin group, and amrinone combined with aprotinin group; each group consisted of eight cases. In the first aprotinin group, 3 x 10(6) units of aprotinin was added to the priming solution of the extracorporeal circulation (ECC). In the second aprotinin group, 3 x 10(6) units of aprotinin was added to the priming solution of ECC, which was combined with 1 x 10(6) units of aprotinin for intravenous bolus. In the amrinone combined with aprotinin group, 3 x 10(6) units aprotinin was added to the priming solution of the ECC and amrinone began with a bolus of 1 mg/kg followed by a maintenance intrusion of 8 micrograms/kg.min. The control group received an equivalence without aprotinin. Venous blood samples were drawn before the operation, at the end of the ECC, 1 hour after the end of the ECC, and one day after the operation, respectively. Flow cytometry was used to demonstrate the expression of leukocyte adhesion molecule CD11b/CD18.

Results: Before ECC, there were no differences of the levels of CD11b+/CD18+ in all groups (P > 0.05). One hour after the ECC, the level of CD11b+/CD18+ in group B was lower than that in group A, the level of CD11b+/CD18+ in group C was lower than that in group B, and the level of CD11b+/CD18+ in group D was higher than that in group C, but no significant difference between groups was noted (P > 0.05).

Conclusion: Although amrinone and aprotinin have anti-inflammatory activity, the pump prime which was added aprotinin alone or aprotinin combined with amrinone might fail in preventing the expression of leukocyte adhesion molecule CD11b/CD18 completely in patients with prosthetic valve replacement during ECC perioperative period.

Objective: This study was designed to observe the genotype and allele frequency of angiotensinogen (AGT) and angiotensin-I converting enzyme (ACE) genes in pregnancy induced hypertension (PIH) and compare them with those in normal pregnant women.

Methods: Eighty-one women with pre-eclampsia (median age 28 years) and 205 normal pregnant women as controls (median age 28 years) from a population of Chinese Han nationality in Chengdu area participated in this study. The pregnant patients whose blood pressure exceeded 140/90 mmHg (or 18.7/12 kPa) were recruited with a rigorous definition of pre-eclampsia. Genotyping was performed using PCR-amplified DNA fragment for ACE I/D and PCR-RFLP for AGT polymorphism.

Results: The allele frequencies of AGT235T and ACE D in PIH and normal pregnant women were 0.856 vs. 0.846 and 0.333 vs 0.364, respectively. No significant difference in these allele frequencies was observed between PIH and normal pregnant women. The allele frequencies of ACE D in Caucasians were higher than those in Chinese population (P < 0.001). The allele frequencies of AGT235T in Caucasians (0.350-0.482) and other populations (0.620-0.739) were lower than that in Chinese (0.846) (P < 0.01, P < 0.001).

Conclusion: There was no evidence in this study for the association of AGT M235T and ACE I/D polymorphism with pre-eclampsia in Chinese population in Chengdu area.

Objective: To investigate the effect of Acanthopanax Giraldii Harms Var Hispidus Hoo polysaccharides (AGP-I) on the human gastric cancer cell.

Methods: DNA agarose gel electrophoresis and flow cytometry techniques were adopted.

Results: After treatment with AGP-I, apoptotic peaks in cell cycle analysis and DNA ladder on the agarose gel were observed.

Conclusion: The results indicate that AGP-1 may inhibit cancer by inducing cancer cell apoptosis.

Objective: To explore the relation between insulin resistance(IR) and A1166C molecular variant of type 1 angiotensin II receptor(AT1R) gene in Chinese subjects with coronary heart disease(CHD).

Methods: IR was calculated by 1/(fast plasma insulin x fast plasma glucose). The A1166C molecular variant of AT1R gene was determined by polymerase chain reaction and restriction endonuclease analysis methods in 90 patients with coronary heart disease and 80 healthy people.

Results: The frequency of allele C of group CHD was significantly higher than that of control group. There was no significant difference of IR level among AT1R A1166C AA, AC, CC genotypes in patients with CHD(P > 0.05).

Conclusion: No significant association between A1166C molecular variant of AT1R gene and insulin resistance was found in Chinese with CHD.

Objective: In order to search for more effective treatment regimen of chronic myeloid leukemia (CML).

Methods: Semisolid culture and conventional chromosome assay were used to evaluate the effect of interferon (IFN) and Ara-c on K562 cell line and BMMNCs from CML patients and normal controls.

Results: IFN had no obvious inhibitory effect on K562 cell. IFN and Ara-c had preferential inhibitory effect on CFU-GM growth of CML and selective cytotoxic effect on CML Ph+ cell. The combination of IFN with Ara-c enhanced the cytotoxic effect of IFN or Ara-c alone on CML cells.

Conclusion: IFN and Ara-c can be selected for therapy of CML in chronic phase. The combination of IFN with Ara-c may be more effective than IFN or Ara-c alone for the therapy of CML.

Objective: To study the effect of a calcium channel antagonist--Nifedipine(NIF) on the prevention and treatment of premature labor, the suppression of uterine contraction, the prolongation of pregnancy, and to observe the side effects of NIF.

Methods: Eighty-four patients with threatened premature labor were randomly divided into three groups according to the dosage of NIF. Group A received NIF according to a 10 mg dosing schedule; Group B received NIF according to a 20 mg dosing schedule; Group C(not treated with NiF) served as control. The time of delivery delayed, the relationship of therapeutic effect with cervical dilatation and gestational age, and the results of delivery were observed and analyzed.

Results: In this study, INF effectively suppressed uterine contraction and delayed the time of delivery. The suppressant intensity was associated with the dosage. The therapeutic effects was apparently more noticeable when the treatment was initiated before the active opening of cervical os. No side effect of NIF was observed in Groups A and B.

Conclusion: Nifedipine is highly effective as a tocolytic agent for the management of premature delivery. It is of clinical usefulness, and the results of the above observations merit further study.

Objective: To investigate osteocalcin and type I collagen mRNA expression of rat marrow stromal cells under induction condition.

Methods: Cultured rat marrow stromal cells were induced by osteogenic compound(composed of dexamethasone 10(-3) mol/L, beta glyceralphosphate 10 mmol/L, ascorbic acid 50 micrograms/ml). Osteocalcin and type I collagen mRNA expression were measured using RT-PCR technique.

Results: It was found that rat marrow stromal cells in induced group have an increased time-dependent expression of osteocalcin and type I collagen mRNA, in comparison with control groups. And the necessity of 1,25-(OH)2D3 in induced osteocalcin mRNA expression was confirmed.

Conclusion: The cultured rat marrow stromal cells kept the undifferentiated state and had osteogenic potential testified by increased osteocalcin and type I collagen mRNA expression when induced.

Unlabelled: To investigate the possible significance and usefulness of reseeding the endothelial cell of recipient for the prevention of hyperacute rejection (HAR) resulting from the xenograft.

Methods: Discordant xenotransplantation model (guinea pig-to-rat) was adopted in this study. Firstly endothelial cells from rat abdominal aorta were separated and cultured. Secondly the guinea pig abdominal aorta of which the endothelium had been removed was cultured with the suspension containing rat endothelial cells (4 x 10(6)/ml). The viability of cultured vessel was assessed using light microscopy and transmission electron microscopy. Thirdly, the guinea pig vessels reseeded with rat endothelial cells were transplanted to rat in vivo. The thrombrosis time was observed. The condition of deposit of IgM and C3 in the recipient's transplanted vessel endothelium was examined by immuno-fluorescence staining assay.

Results: The thrombrosis time of the guinea pig vessel reseeded with rat endothelial cells (20.3 + 4.42 h) was significantly prolonged as compared with that of the normal guinea pig vessel (0.35 + 0.284 h) and the guinea pig vessel that had been deprived of endothelium (0.165 + 0.77 h). No deposit of IgM and C3 was seen in the new endothelium of guinea pig vessel in the treated group, whereas deposit of IgM and C3 was observed in the untreated normal guinea pig aorta.

Conclusion: Donor vessels reseeded with endothelial cells from the recipient will undergo less severe rejection and this technique may be very useful in the attenuation of HAR.