[Construction of DNA vaccine including a chimeric gene encoding flagellin and outer membrane protein antigen from Leptospira interrogons serovar lai].

Abstract

Objective: To construct a fusion expression vector for DNA vaccine, including a flagellin gene (flaB2) and an outer membrane protein gene (ompL1) to enhance the protective immunity of Leptospira interrogans serova Lai (Stain 017).

Methods: The DNA fragments encoding flaB2 and ompL1 were amplified respectively by PCR and then fused. The obtained chimeric gene ompL1-flaB2 contained a synthetic linker of ten amino acids to sustain its space conformation.

Results: Identified by restriction enzyme analysis, an insert fragment of 1.8 kb was demonstrated. FlaB2 and ompL1 respectively had the same sequence as reported by DNA sequencing analysis.

Conclusion: A fusion expression plasmid containing flaB2 and ompL1 was constructed.