{"title":"[Comparison of the immune response to gene transfer into the rat brain with helper or helper virus-free HSV-1 vectors].","authors":"Wei Zhang, A Geller","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to compare the immune response to gene transfer with helper virus or helper virus-free vectors.</p><p><strong>Methods: </strong>Two vector stocks were delivered by stereotactic injection into the striatum and then the immune response markers including TCR, CD4, CD8, MHC-I and MHC-II were examined.</p><p><strong>Results: </strong>The data showed that the immune response to either vector stock reached maximal levels by 4 to 7 days after gene transfer. However, the maximal levels of all markers to the helper virus-free stock were 3 to 10 folds lower than the markers to the helper virus stock. The immune response to the helper virus-free stock declined rapidly to be close to the levels of PBS control.</p><p><strong>Conclusion: </strong>The immune response to the helper virus-free stock is significantly less than the response to the vector stock that contains helper virus.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"175-8"},"PeriodicalIF":0.0000,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: This study was conducted to compare the immune response to gene transfer with helper virus or helper virus-free vectors.
Methods: Two vector stocks were delivered by stereotactic injection into the striatum and then the immune response markers including TCR, CD4, CD8, MHC-I and MHC-II were examined.
Results: The data showed that the immune response to either vector stock reached maximal levels by 4 to 7 days after gene transfer. However, the maximal levels of all markers to the helper virus-free stock were 3 to 10 folds lower than the markers to the helper virus stock. The immune response to the helper virus-free stock declined rapidly to be close to the levels of PBS control.
Conclusion: The immune response to the helper virus-free stock is significantly less than the response to the vector stock that contains helper virus.
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