[Gene expression of insulin-like growth factors in human villous trophoblast cells of early pregnancy in vitro].

Abstract

Objective: To investigate the gene expression of insulin-like growth factors(IGFs) in human villous trophoblast cells of early pregnancy in vitro and their action.

Methods: Semiquantitative reverse transcriptase polymerase chain reaction(RT-PCR), with beta-ACTIN as internal standard, was applied to determine the expression of IGFs messenger RNA in human villous trophoblast cells of early pregnancy in vitro.

Results: The expression of IGF-II mRNA, IGF-I R mRNA and IGFBP-3 mRNA was detectable in human villous trophoblast cells of early pregnancy in vitro.

Conclusion: IGFs appear to play important regulation roles in early invasion, proliferation and differentiation of cytotrophoblast, and in the formation of placenta and the development of embryo via autocrine and/or paracrine way.