[Generation of dendritic cells from healthy human peripheral blood].

Abstract

Objective: To get high quality and sufficient numbers of mature dendritic cells (DC) from healthy human peripheral blood.

Methods: Cultured plastic-adherent monocytes were isolated from healthy human peripheral blood by use of granulocyte-monocyte clony-stimulating factor and Interleukin-4 for 7 days without fed with fresh medium and cytokine.

Results: A large number of DCs with high purity were generated. These DCs expressed HLA-I, II molecules, Costimulating molecules and adherent molecules highly, showing the characteristics of mature DC. These DCs could stimulate proliferation of allogenic T lymphocytes and had active endocytosis ability which peaked at the third day in culture but decreased afterwards.

Conclusion: These results provide evidence that CD monocytes isolated from peripheral blood monocytes exhibit dendritic cells characteristics and may facilitate further studies of DC and its clinical application.