[Simultaneous determination of 13 polycyclic aromatic hydrocarbons in indoor air by HPLC].

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2002-01-01

Deyun Zhang, Chengjun Sun, Tao Wang

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Abstract

Objective: To establish a method for the simultaneous determination of 13 polycyclic aromatic hydrocarbons (PAHs) in indoor air by high performance liquid chromatography (HPLC) using methanol/water as mobile phase.

Methods: The analytical procedure involves collecting indoor airborne PAHs on quartz fiber filter, ultrasonic extraction with methanol, transferring PAHs to methanol after blowing off the solvent with nitrogen gas followed with separation and quantitation with reverse-phase HPLC using gradient elution and fluorescence programmed multiple wavelength shift detection. The separation and determination parameters were optimized. PAHs were spiked to evaluate the accuracy of the method.

Results: The detection limits of 13 PAHs varied from 0.11 to 11.7 pg. The recoveries ranged between 80.7% and 112.1%. The within-day (n = 5) and between-day (n = 6) relative standard deviations were 0.5%-5.5% and 2.4%-9.1% respectively.

Conclusions: This method is rapid, economical, accurate, and suitable for determining the trace PAHs in indoor air.

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[HPLC法同时测定室内空气中13种多环芳烃]。

目的:建立以甲醇/水为流动相的高效液相色谱法同时测定室内空气中13种多环芳烃(PAHs)的方法。方法:采用石英纤维过滤器采集室内空气中多环芳烃，甲醇超声提取，氮气吹除溶剂后将多环芳烃转移至甲醇，反相高效液相色谱梯度洗脱分离定量，荧光程序多波长移位检测。对分离测定参数进行了优化。加入多环芳烃以评估该方法的准确性。结果:13种PAHs的检出限为0.11 ~ 11.7 pg，加样回收率为80.7% ~ 112.1%。日内(n = 5)和日间(n = 6)的相对标准偏差分别为0.5% ~ 5.5%和2.4% ~ 9.1%。结论:该方法快速、经济、准确，适用于室内空气中痕量多环芳烃的测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao

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