Evidences of differential methylation in the genome during development in the cactophilic Drosophila species

IF 2.4 4区生物学 Q2 DEVELOPMENTAL BIOLOGY genesis Pub Date : 2023-09-26 DOI:10.1002/dvg.23554

Adriano S. Santos, Ester S. Ramos, Vera L. S. Valente-Gaiesky, Fábio de Melo Sene, Maura H. Manfrin

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Abstract

DNA methylation with 5-methylcytosine (5mC) has been reported in the genome of several eukaryotes, with marked differences between vertebrates and invertebrates. DNA methylation is poorly understood as its role in evolution in insects. Drosophila gouveai (cluster Drosophila buzzatii) presents larvae that develop obligatorily in necrotic tissues of cacti in nature, with the distribution of populations in South America, and plasticity of phenotypes in insect–plant interaction. We characterize organisms at developmental stages and analyze variations at multiple methylation-sensitive loci in pupae, and adult flies using methylation sensitive amplification polymorphism. We obtained 326 loci with CCGG targets in the genome of D. gouveai. Genomic regions with molecular lengths from 100 to 700 pb were most informative about methylation states. Multiple loci show differences in methylation-sensitive sites (MSL) concerning developmental stages, such as in pupae (MSL = 40), female reproductive tissue (MSL = 76), and male reproductive tissues (MSL = 58). Our results are the first evidence of genome-wide methylation in D. gouveai organisms.

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亲仙人掌果蝇发育过程中基因组差异甲基化的证据。

在几种真核生物的基因组中已经报道了5-甲基胞嘧啶（5mC）的DNA甲基化，脊椎动物和无脊椎动物之间存在显著差异。DNA甲基化在昆虫进化中的作用尚不清楚。gouveai果蝇（集群Drosophila buzzatii）在自然界中呈现出在仙人掌坏死组织中强制发育的幼虫，具有南美洲种群的分布和昆虫与植物相互作用表型的可塑性。我们对处于发育阶段的生物体进行了表征，并使用甲基化敏感扩增多态性分析了蛹和成虫中多个甲基化敏感基因座的变异。我们在小牛肉基因组中获得了326个CCGG靶位点。分子长度从100到700 pb的基因组区域对甲基化状态的信息最多。多个基因座显示出与发育阶段有关的甲基化敏感位点（MSL）的差异，例如在蛹中（MSL = 40），雌性生殖组织（MSL = 76）和雄性生殖组织（MSL = 58）。我们的研究结果是第一个在D.gouveai生物体中全基因组甲基化的证据。

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来源期刊

genesis 生物-发育生物学

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： As of January 2000, Developmental Genetics was renamed and relaunched as genesis: The Journal of Genetics and Development, with a new scope and Editorial Board. The journal focuses on work that addresses the genetics of development and the fundamental mechanisms of embryological processes in animals and plants. With increased awareness of the interplay between genetics and evolutionary change, particularly during developmental processes, we encourage submission of manuscripts from all ecological niches. The expanded numbers of genomes for which sequencing is being completed will facilitate genetic and genomic examination of developmental issues, even if the model system does not fit the “classical genetic” mold. Therefore, we encourage submission of manuscripts from all species. Other areas of particular interest include: 1) the roles of epigenetics, microRNAs and environment on developmental processes; 2) genome-wide studies; 3) novel imaging techniques for the study of gene expression and cellular function; 4) comparative genetics and genomics and 5) animal models of human genetic and developmental disorders. genesis presents reviews, full research articles, short research letters, and state-of-the-art technology reports that promote an understanding of the function of genes and the roles they play in complex developmental processes.