Effect of NaCl Stress on the Anatomical Structure and Ultrastructure of Leaves of Berberis thunbergii var. atropurpurea and Berberis thunbergii ‘bingchuanhongye’

Yan Li, B. Lu, Yufei Zhu, Bing-she Lu
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Abstract

In this study, we first investigated the effects of different concentrations of NaCl stress treatments (0%, 0.1%, 0.2%, 0.3%, and 0.4%) on the anatomical structure and ultrastructure of plant leaves using annual seedlings of Berberis thunbergii var. atropurpurea and Berberis thunbergii ‘bingchuanhongye.’ To provide a detailed analysis, scanning and transmission electron microscopy was utilized. Scanning electron microscopy indicated that the increased NaCl stress concentration decreased the number of stomatal openings, open stomatal percentage, and stomatal length, width, and size of the lower epidermis of both Berberis species. The epidermal cells and stomata of Berberis thunbergii ‘bingchuanhongye’ were significantly wrinkled and damaged, while the arrangement of the palisade tissue and spongy tissue of Berberis thunbergii var. atropurpurea was more orderly than that of Berberis thunbergii ‘bingchuanhongye.’ Transmission electron microscopy showed that the cytoplasm of Berberis thunbergii var. atropurpurea gradually shrank and exhibited serious plasmolysis with the increase of NaCl stress concentration. At the same time, the chloroplasts became larger and deformed, and the number of mitochondria, osmiophilic granules, and starch granules gradually increased. In contrast, the inward contraction of the protoplasm of Berberis thunbergii ‘bingchuanhongye’ was not obvious, the chloroplasts were smaller and fewer in number, and the number of mitochondria, osmiophilic granules, and starch granules gradually decreased until degradation. This study not only preliminarily determined that Berberis thunbergii var. atropurpurea showed slightly higher salt tolerance than Berberis thunbergii ‘bingchuanhongye,’ but also provided insights into their response mechanisms to saline environments.
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NaCl胁迫对东北小檗和冰川红叶小檗叶片解剖结构和超微结构的影响
在本研究中,我们首先研究了不同浓度的NaCl胁迫处理(0%、0.1%、0.2%、0.3%和0.4%)对东北小檗和东北小檗的年生幼苗叶片解剖结构和超微结构的影响为了提供详细的分析,使用了扫描和透射电子显微镜。扫描电子显微镜显示,NaCl胁迫浓度的增加降低了两种小檗属植物下表皮的气孔数量、气孔开放率以及气孔长度、宽度和大小。冰川红叶小檗表皮细胞和气孔明显皱折和损伤,而萎缩小檗栅栏组织和海绵组织的排列比冰川红叶小檗整齐透射电镜显示,随着NaCl胁迫浓度的增加,浙小檗的细胞质逐渐缩小,并表现出严重的胞浆分裂。与此同时,叶绿体变大变形,线粒体、嗜锇颗粒和淀粉颗粒的数量逐渐增加。相反,浙江小檗“冰川红叶”原生质体向内收缩不明显,叶绿体越来越小,线粒体、嗜锇颗粒和淀粉颗粒的数量逐渐减少,直至降解。本研究不仅初步确定了东北小檗的耐盐性略高于冰川红叶小檗,而且为其对盐碱环境的反应机制提供了见解。
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
自引率
0.00%
发文量
60
审稿时长
6 months
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