Pituitary-Thyroid System in Rats of Different Ages Under Desynchronosis, Whole-Body Cryostimulation, and Cord Blood Injection

Q4 Medicine Problems of Cryobiology and Cryomedicine Pub Date : 2022-09-30 DOI:10.15407/cryo32.03.196

V. Lomako, O. Shylo, L. Samokhina, D. Lutsenko

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Abstract

The state of the pituitary-thyroid system central and peripheral links (according to the levels of thyroid stimulating hormone (TSH) and thyroid hormones T4 and T3 in blood serum) were studied in 6- and 18-month-old rats under initiated circadian desynchronosis (CD) by the light regime inverting. The CD was simulated against the background of separate and combined eff ect of both the whole body cryostimulation (–120°C) (WBC) and injections of the nucleated cells (NCs) of human cord blood, applied preventively (for a day and week, respectively). In 6-month-old rats, the TSH level decreased under the CD and its initiation after separate NCs administration and combined with WBC (by 3.4, 3.9 and 6.3 times, respectively) and remained unchanged under CD against the WBC. In 18-month-old rats the TSH level was reduced only at CD by 1.8 times. The T4 level in rats of both age groups did not change under the CD as well as during its development against the background of a combined effect of WBC and NCs administration in 6-month-old animals and against the NCs injection in 18 months-old rats. The T4 level in rats of both age groups decreased at the background of separate effect of the WBC (by 1.6 and 1.4 times, respectively). In 18-month-old animals, the T4 increased under the CD development after a combined effect of WBC and NCs injection by 1.3 times. No changes in the T3 level were found.

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不同年龄大鼠垂体-甲状腺系统在失同步、全身冷冻刺激和脐带血注射下的变化

采用光照逆转法研究了6月龄和18月龄大鼠在初始性昼夜节律不同步(CD)下垂体-甲状腺系统中枢和外周环节的状态(根据血清中促甲状腺激素(TSH)和甲状腺激素T4和T3的水平)。在全身冷冻刺激(-120°C)和预防性注射人脐带血有核细胞(NCs)单独和联合作用的背景下模拟CD(分别为1天和1周)。在6个月大的大鼠中，TSH水平在分别给予nc和WBC后分别降低了3.4倍、3.9倍和6.3倍，并在CD对抗WBC时保持不变。在18个月大的大鼠中，TSH水平仅在CD时降低了1.8倍。两个年龄组大鼠的T4水平在CD下以及在其发育过程中没有变化，背景是6月龄大鼠的WBC和NCs联合施用以及18月龄大鼠的NCs注射。在白细胞单独作用的背景下，两组大鼠的T4水平分别下降1.6倍和1.4倍。在18月龄的动物中，WBC和NCs联合作用后，T4在CD发育过程中增加了1.3倍。T3水平未见变化。

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

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期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.