Morphological changes of the brain cortex sensorimotor area in 6-month-old rats were studied after short-term intermittent and long-term continuous cold exposures (STICE and LTCCE, respectively). Under these conditions, perivascular and pericellular edema, small solitary foci of necrosis with gliosis phenomena, "porous" neuropil (signs under STICE at –12°С and formation during LTCCE), dystrophically changed neurons, neurons with nuclei at different stages of necrobiosis were noted in the brain tissue against the background of native cyto- and myeloarchitectonics, Some of the microvessels had signs of inflammation and thrombi. A moderate constriction (after STICE at –12°С) and a dilatation (after STICE at 10°С) of microvessels were observed. According to the fractal analysis, a decrease in the fractal dimension of the brain cortex sections was found under both STICE at –12°С and LTCCE.
{"title":"Brain Cortex Morphology in Rats After Cold Exposures","authors":"V. Lomako, O. Shylo, L. Pirozhenko","doi":"10.15407/cryo33.03.193","DOIUrl":"https://doi.org/10.15407/cryo33.03.193","url":null,"abstract":"Morphological changes of the brain cortex sensorimotor area in 6-month-old rats were studied after short-term intermittent and long-term continuous cold exposures (STICE and LTCCE, respectively). Under these conditions, perivascular and pericellular edema, small solitary foci of necrosis with gliosis phenomena, \"porous\" neuropil (signs under STICE at –12°С and formation during LTCCE), dystrophically changed neurons, neurons with nuclei at different stages of necrobiosis were noted in the brain tissue against the background of native cyto- and myeloarchitectonics, Some of the microvessels had signs of inflammation and thrombi. A moderate constriction (after STICE at –12°С) and a dilatation (after STICE at 10°С) of microvessels were observed. According to the fractal analysis, a decrease in the fractal dimension of the brain cortex sections was found under both STICE at –12°С and LTCCE.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139333074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Kudokotseva, I. Lomakin, V. Babiichuk, L. Babiichuk, Igor Kandybko
The paper presents the research results of spatial memory and learning processes for the comprehensive analysis of cognitive function of spontaneously hypertensive rats under the influence of independent and combined use of rhythmic craniocerebral hypothermia (RCH) and the introduction of cryopreserved cord blood nucleated cells (cCBNCs). It was shown that SHR rats have a cognitive deficit, which is manifested in reduced speed of the learning process and memory formation in the Morris water maze (MWM). The administration of therapeutic RCH and/or cell therapy (cCBNCs introduction) 30 days before training in the MWM improved the cognitive functions of SHR rats: the target search strategy changed, and the time to search for a platform hidden under water was significantly reduced. These changes indicated the acceleration of the processes of information perception, learning, and the formation of spatial memory. The preservation and reproduction of long-term memory and as a result of the formed strategy of direct finding of the target in the MWM was most influenced by certain therapy with the samples of cryopreserved cord blood in combination with the procedure of therapeutic hypothermia. It was established that this approach exerted a neuroprotective effect, contributing to the partial elimination of cognitive deficits in SHR by improving spatial memory and accelerating learning processes.
{"title":"Therapeutic Hypothermia and Cell Therapy Change Cognitive Functions of Spontaneously Hypertensive Rats","authors":"O. Kudokotseva, I. Lomakin, V. Babiichuk, L. Babiichuk, Igor Kandybko","doi":"10.15407/cryo33.03.201","DOIUrl":"https://doi.org/10.15407/cryo33.03.201","url":null,"abstract":"The paper presents the research results of spatial memory and learning processes for the comprehensive analysis of cognitive function of spontaneously hypertensive rats under the influence of independent and combined use of rhythmic craniocerebral hypothermia (RCH) and the introduction of cryopreserved cord blood nucleated cells (cCBNCs). It was shown that SHR rats have a cognitive deficit, which is manifested in reduced speed of the learning process and memory formation in the Morris water maze (MWM). The administration of therapeutic RCH and/or cell therapy (cCBNCs introduction) 30 days before training in the MWM improved the cognitive functions of SHR rats: the target search strategy changed, and the time to search for a platform hidden under water was significantly reduced. These changes indicated the acceleration of the processes of information perception, learning, and the formation of spatial memory. The preservation and reproduction of long-term memory and as a result of the formed strategy of direct finding of the target in the MWM was most influenced by certain therapy with the samples of cryopreserved cord blood in combination with the procedure of therapeutic hypothermia. It was established that this approach exerted a neuroprotective effect, contributing to the partial elimination of cognitive deficits in SHR by improving spatial memory and accelerating learning processes.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cryopreservation of human oocytes by the vitrification has become a part of the daily practice of assisted reproductive technologies to fight an infertility and to preserve the woman's reproductive potential. This literature review covers the results of the studies performed by various scientific schools during 25 years; historical prerequisites for the development of cryopreservation of human oocytes by vitrification; medical and social indices to apply this method, features and complications arising during vitrification of human oocytes. Special attention has been paid to the issues regarding the genetic safety of the method mentioned in respect of the health of future descendants. The paper presents the insight on unsolved tasks in vitrification of oocytes and that of identified ways to overcome the challenges.
{"title":"Quarter-Century Experience in Cryopreservation of Human Oocytes by Vitrifi cation. What Has Been Achieved and What is Next?","authors":"T. Yurchuk, M. Petrushko","doi":"10.15407/cryo33.03.177","DOIUrl":"https://doi.org/10.15407/cryo33.03.177","url":null,"abstract":"Cryopreservation of human oocytes by the vitrification has become a part of the daily practice of assisted reproductive technologies to fight an infertility and to preserve the woman's reproductive potential. This literature review covers the results of the studies performed by various scientific schools during 25 years; historical prerequisites for the development of cryopreservation of human oocytes by vitrification; medical and social indices to apply this method, features and complications arising during vitrification of human oocytes. Special attention has been paid to the issues regarding the genetic safety of the method mentioned in respect of the health of future descendants. The paper presents the insight on unsolved tasks in vitrification of oocytes and that of identified ways to overcome the challenges.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Kolomatska, Liana Liana, Valentyna Syvenko, Nina Leonova
{"title":"Can Cold-Tolerant Hybrids Be Created by Crossing Cold-Tolerant Parental Lines?","authors":"V. Kolomatska, Liana Liana, Valentyna Syvenko, Nina Leonova","doi":"10.15407/cryo33.03.212","DOIUrl":"https://doi.org/10.15407/cryo33.03.212","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139333054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The review surveys the published data on a strong negative impact of psycho-emotional stress, which accompanies both the military servicemen and civilians of any state in martial law period. Such a load can lead to severe disorders of the systems that ensure the human body homeostatic stability, in particular to post-traumatic stress disorder. In this case human body is subjected to a state accompanied by the development of severe oncology and autoimmune diseases, as well as dysfunction of the male reproductive system. The study offers modern approaches to the treatment of the listed pathologies, most of which are based on the international experience of clinical and experimental research as well as achievements of national academic science. Special attention is paid to the development of cryobiological and cryomedical cell-tissue techniques, the use of those can be promising in the strengthening of both mental and physical health of civilian population during the wartime.
{"title":"Cryobiology and Cryomedicine Techniques’ Boosting in Wartime","authors":"Anatoliy Goltsev, Mykola Bondarovych, Nataliia Volkova, Olena Lutsenko, Yuliia Gaevska, Natalia Babenko, Olha Chelombytko, Maksym Ostankov, Dmytro Vvedenskyi, Hanna Kisielova, Tetiana Dubrava","doi":"10.15407/cryo33.02.077","DOIUrl":"https://doi.org/10.15407/cryo33.02.077","url":null,"abstract":"The review surveys the published data on a strong negative impact of psycho-emotional stress, which accompanies both the military servicemen and civilians of any state in martial law period. Such a load can lead to severe disorders of the systems that ensure the human body homeostatic stability, in particular to post-traumatic stress disorder. In this case human body is subjected to a state accompanied by the development of severe oncology and autoimmune diseases, as well as dysfunction of the male reproductive system. The study offers modern approaches to the treatment of the listed pathologies, most of which are based on the international experience of clinical and experimental research as well as achievements of national academic science. Special attention is paid to the development of cryobiological and cryomedical cell-tissue techniques, the use of those can be promising in the strengthening of both mental and physical health of civilian population during the wartime.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136366743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Protective Media Composition and Freezing Regimen Experimental Substantiation During Mycobacterium Avium Freeze-Drying","authors":"Andrii Zavhorodnii, Viktor Bilushko, Mykola Kalashnyk, Svitlana Pozmogova, Anatoliy Paliy, Olena Pavlichenko","doi":"10.15407/cryo33.02.139","DOIUrl":"https://doi.org/10.15407/cryo33.02.139","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136366756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanna Kisielova, Tetiana Dubrava, Anatoliy Goltsev
{"title":"Heat Shock Proteins as Key Triggers to Form Cryopreserved Progenitors-Derived Tolerogenic Dendritic Cells","authors":"Hanna Kisielova, Tetiana Dubrava, Anatoliy Goltsev","doi":"10.15407/cryo33.02.144","DOIUrl":"https://doi.org/10.15407/cryo33.02.144","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136366755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The paper presents experimental data on the determination of preservation rate of human cord blood nucleated cells and their apoptosis/necrosis stages to determine the number of viable functionally active cells after cryopreservation in solutions with different concentrations of DMSO and the water-soluble analogue of vitamin E, the antioxidant trolox. Counting the number of human cord blood nucleated cells after freezing in media with the addition of trolox revealed their maximum preservation in the samples with 7.5% DMSO and 50, 70 or 200 μM of the antioxidant. Using the flow cytometry with the addition of the Annexin V FITC reagent, which specifically binds to phospholipids, and the DNA dye 7-amino-actinomycin D (7-AAD), it was established that trolox in concentrations of 50–70 μM provided an increase in the number of viable cells with intact membrane (AnnexinV─/7AAD─) by 12–16% compared to the control, which involved the use of only DMSO in the cryoprotective solution. The obtained results indicate the effectiveness of using the antioxidant trolox and the prospects of developing trolox-containing cryoprotective mixtures for freezing and long-term storage of nucleated cord blood cells, including hematopoietic progenitor cells.
{"title":"Trolox Antioxidant as a Factor in Stabilization of Human Cord Blood Nucleated Cells During Cryopreservation","authors":"Pavlo Zubov, Oksana Zubova, Lyubov Babijchuk","doi":"10.15407/cryo33.02.122","DOIUrl":"https://doi.org/10.15407/cryo33.02.122","url":null,"abstract":"The paper presents experimental data on the determination of preservation rate of human cord blood nucleated cells and their apoptosis/necrosis stages to determine the number of viable functionally active cells after cryopreservation in solutions with different concentrations of DMSO and the water-soluble analogue of vitamin E, the antioxidant trolox. Counting the number of human cord blood nucleated cells after freezing in media with the addition of trolox revealed their maximum preservation in the samples with 7.5% DMSO and 50, 70 or 200 μM of the antioxidant. Using the flow cytometry with the addition of the Annexin V FITC reagent, which specifically binds to phospholipids, and the DNA dye 7-amino-actinomycin D (7-AAD), it was established that trolox in concentrations of 50–70 μM provided an increase in the number of viable cells with intact membrane (AnnexinV─/7AAD─) by 12–16% compared to the control, which involved the use of only DMSO in the cryoprotective solution. The obtained results indicate the effectiveness of using the antioxidant trolox and the prospects of developing trolox-containing cryoprotective mixtures for freezing and long-term storage of nucleated cord blood cells, including hematopoietic progenitor cells.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136366748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sensitivity of microbial cultures to antibiotics was determined using disco-diffusion method, broth serial dilution and agar serial dilution. Restored viability and detection of antibiotic resistance in the museum Escherichia coli strains stored for a lasting time in a lyophilized state indicates that freeze-drying in combination with moderately low temperatures for subsequent long-term storage is an eff ective method of maintaining collection microorganism strains in a functionally stable state, including preservation of their antibiotic resistance. It was found that one of the 21 studied strains was resistant to ampicillin, ampicillin-sulbactam, cephalosporins of the I–III generations, two strains were resistant to chloramphenicol, and four strains were insensitive to tetracycline. Detection of the resistance to cephalosporins and protected penicillins in E. coli strain isolated in 1963, long before the discovery and use of these antibiotics in clinical practice, confirms that antibiotic resistance is a phenomenon associated rather with the general mechanisms of adaptation of pathogens to adverse external environmental factors than with the widespread use of antimicrobial drugs.
{"title":"Antibiotic Sensitivity of Escherichia Coli Museum Strains After Long-Term Storage in a Lyophilized State at Moderately Low Temperatures","authors":"Yulia Yagnuk, Tetiana Gurina, Olena Peretyatko, Nadia Sklyar, Аndrii Yagnuk, Svitlana Kalinichenko","doi":"10.15407/cryo33.02.115","DOIUrl":"https://doi.org/10.15407/cryo33.02.115","url":null,"abstract":"Sensitivity of microbial cultures to antibiotics was determined using disco-diffusion method, broth serial dilution and agar serial dilution. Restored viability and detection of antibiotic resistance in the museum Escherichia coli strains stored for a lasting time in a lyophilized state indicates that freeze-drying in combination with moderately low temperatures for subsequent long-term storage is an eff ective method of maintaining collection microorganism strains in a functionally stable state, including preservation of their antibiotic resistance. It was found that one of the 21 studied strains was resistant to ampicillin, ampicillin-sulbactam, cephalosporins of the I–III generations, two strains were resistant to chloramphenicol, and four strains were insensitive to tetracycline. Detection of the resistance to cephalosporins and protected penicillins in E. coli strain isolated in 1963, long before the discovery and use of these antibiotics in clinical practice, confirms that antibiotic resistance is a phenomenon associated rather with the general mechanisms of adaptation of pathogens to adverse external environmental factors than with the widespread use of antimicrobial drugs.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136366758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nadiia Shevchenko, Olena Terekhova, Anna Kaverinska, Olga Prokopiuk, Viktoriya Lazurenko, Volodymyr Prokopіuk
{"title":"Evaluation of Effectiveness of Umbilical Cord Cryoextract in Aesthetic Medicine (in Vitro Study)","authors":"Nadiia Shevchenko, Olena Terekhova, Anna Kaverinska, Olga Prokopiuk, Viktoriya Lazurenko, Volodymyr Prokopіuk","doi":"10.15407/cryo33.02.133","DOIUrl":"https://doi.org/10.15407/cryo33.02.133","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136366749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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