Lyophilized Human Cord Blood Leukoconcentrate to Treat Brain Ischemia in Rats

Q4 Medicine Problems of Cryobiology and Cryomedicine Pub Date : 2022-03-30 DOI:10.15407/cryo32.01.044

V. Lebedinets, L. Ostankova, M. Bondarovych, O. Lutsenko, D. Lebedinets, Igor Grisha, M. Ostankov, K. Parkhomenko, A. Goltsev

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引用次数: 2

Abstract

The paper outlines the experimental data on the impact of lyophilization on preservation of structural and functional characteristics of cells from human cord blood leukoconcentrate (HCBL). Lyophilization of HCBL was shown to ensure the preservation of cells with immune modulating potential in a heterogeneous population of HCBL. Lyophilized HCBL (lHCBL), to the same extent as cryopreserved one (cHCBL), revealed an equal immune correcting effect during ischemic brain injury in vivo in the experimental model of ischemic stroke (IS). The inclusion of lHCBL in therapy of brain ischemia demonstrated the improved indices of IFN-ү+ and IL-10+ spleen cells, adhesive and phagocytic activity of peritoneal cavity cells in rats with IS. The efficiency of using both lHCBL and cHCBL during IS may be associated with the implementation of therapeutic effect by presented among them cells and mediators, including multi-vector regulatory systems that maintain homeostatic stability of the body (immune, endocrine, nervous ones).

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冷冻人脐血浓缩白细胞治疗大鼠脑缺血

本文概述了冷冻干燥对保存人脐血白细胞浓缩物（HCBL）细胞结构和功能特征的影响的实验数据。HCBL的冷冻被证明可以确保在异质性的HCBL群体中保存具有免疫调节潜力的细胞。在缺血性脑卒中（IS）的实验模型中，冻干的HCBL（lHCBL）在体内缺血性脑损伤期间显示出与冷冻保存的HCBL相同的免疫校正作用。将lHCBL纳入脑缺血治疗中，可改善IS大鼠脾细胞IFN-119和IL-10+的指标、腹腔细胞的粘附和吞噬活性，包括维持身体稳态稳定的多载体调节系统（免疫、内分泌、神经系统）。

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.