Oleksandr Osetskyi, T. Gurina, Anna Poliakova, Stanislav Sevastianov
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引用次数: 1
Abstract
For the first time the possibility of using thermomechanical analysis to construct the fragments of state diagrams of cryoprotective solutions in the zone of glass transition temperatures has been considered. A method for studying cluster crystallization of cryoprotective solutions based on thermomechanical curves has been developed. The parameters of thermomechanical curves of frozen aqueous solutions of dimethyl sulfoxide (DMSO), polyethylene oxide 1500 (PEO-1500), glycerol were analyzed and the relationship between these parameters and the cluster crystallization kinetics for these solutions was established. On the basis of experimentally obtained thermomechanical curves for the frozen solutions of DMSO and PEO-1500 the possibility of formation of clusters of two types has been shown: on the basis of ice and cryoprotective substance microcrystals. Additional experimental data were obtained to construct a complete state diagrams of cryoprotective solutions, which include the existing cluster phase areas.
期刊介绍：
The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.
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