Construction of Longan (Dimocarpus longan Lour.) Simple Sequence Repeat Fingerprints and Application in Distinctness, Uniformity and Stability Testing

Q. Ma, Mengqiang Chen, Hong Liu, Liqing Liu, Jiwei Yin, Xi Yang, Jialin Lv, D. Rao, S. Shi, Zhenjiang Xu
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Abstract

Simple sequence repeat (SSR) markers are highly polymorphism, good reproducibility, abundant number and co-dominant inheritance, and were considered to be one of the preferred molecular markers for DNA fingerprints in distinctness, uniformity and stability (DUS) testing. In this study, 10 representative Dimocarpus longan Lour (longan) varieties with significant differences were selected from 63 longan varieties according to the morphological characteristics. Based on PCR amplifications of the 10 selected varieties, 24 SSR primers pairs were screened from total 300 SSR primers pairs, to establish SSR fingerprints for all 63 longan varieties. The results showed that a total of 127 alleles were detected in 63 longan varieties, with an average of 5.29 alleles for each pair of primers. The Shannon’s index of the 24 pairs of SSR markers ranged from 0.64 to 1.58, with an average of 1.20. The polymorphism information content of each locus ranged from 0.32 to 0.72, with an average of 0.58. Clustering analysis indicated that most of the varieties with close genetic relationships tended to fall in the same cluster, and only a few in different clusters or sub-clusters. The 63 longan varieties were completely identified by the optimal combination of 6 pairs of SSR primers (LY161, LY252, LY137, LY130, LY25 and LY34). Overall, DNA fingerprints of the 63 longan varieties were constructed based on these 24 pairs of SSR primers. This study may provide a technical support for variety identification and similar variety screening in DUS detection in longan.
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龙眼(Dimocarpus Longan Lour.)的构造简单序列重复指纹图谱及其在鉴别性、均匀性和稳定性检测中的应用
简单序列重复(SSR)标记具有多态性高、重现性好、数量丰富、共显性遗传等特点,是DNA指纹鉴别、均匀性和稳定性(DUS)检测的首选分子标记之一。本研究根据形态特征,从63个龙眼品种中筛选出10个具有代表性、差异显著的龙眼品种。根据10个选择品种的PCR扩增结果,从300对SSR引物中筛选出24对,建立了63个龙眼品种的SSR指纹图谱。结果表明,63个龙眼品种共检测到127个等位基因,平均每对引物检测到5.29个等位基因。24对SSR标记的香农指数范围为0.64 ~ 1.58,平均为1.20。每个位点的多态性信息含量在0.32 ~ 0.72之间,平均为0.58。聚类分析结果表明,亲缘关系较近的品种多数属于同一聚类,少数属于不同聚类或亚聚类。通过6对SSR引物LY161、LY252、LY137、LY130、LY25和LY34的最优组合,对63个龙眼品种进行了完整的鉴定。利用这24对SSR引物构建了63个龙眼品种的DNA指纹图谱。本研究可为龙眼DUS检测中的品种鉴定和同类品种筛选提供技术支持。
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
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0.00%
发文量
60
审稿时长
6 months
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