Measurement of Sufentanil in Human Milk by Liquid Chromatography-Tandem Mass Spectrometry

Yangfa Huang, Lianming Liao, Manlin Zeng, Qijian Huang, J. Zeng, Liangcheng Zhang
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Abstract

A sensitive quantification method for sufentanil in human milk was developed. Samples were prepared by liquid-phase extraction. Analytes were chromatographically separated. The mobile phase contained 10 mM ammonium formate (A) and methanol (B). The ratios of A to B were 50:50, 10:90, and 50:50 sequentially. For LC-MS-MS detection, transition of the protonated precursor ions to product ions (sufentanil: m/z 387.2→m/z 238.2; internal control fentanil: m/z 337.2→m/z 158.2) was monitored. The linear quantification range of sufentanil concentration was 3.2–400 pg ·mL−1. The correlation coefficient (r2) was 0.996. The % RSD was less than 6.0% for intraday and less than 8.68% for interday precision. By this method, sufentanil contents were determined to be very low in the postnatal maternal milk samples.
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液相色谱-串联质谱法测定母乳中舒芬太尼的含量
建立了人乳中舒芬太尼的灵敏定量方法。采用液相萃取法制备样品。分析物经色谱分离。流动相为10 mM甲酸铵(A)和甲醇(B), A与B的比例依次为50:50、10:90和50:50。lc - ms检测时,质子化前体离子转变为产物离子(舒芬太尼:m/z 387.2→m/z 238.2;内部控制芬太尼:m/z 337.2→m/z 158.2)监测。舒芬太尼浓度线性定量范围为3.2 ~ 400 pg·mL−1。相关系数(r2)为0.996。日内RSD小于6.0%,日内RSD小于8.68%。用该方法测定了产后母乳样品中舒芬太尼的含量很低。
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
自引率
0.00%
发文量
60
审稿时长
6 months
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