{"title":"Measurement of Sufentanil in Human Milk by Liquid Chromatography-Tandem Mass Spectrometry","authors":"Yangfa Huang, Lianming Liao, Manlin Zeng, Qijian Huang, J. Zeng, Liangcheng Zhang","doi":"10.1166/jbmb.2023.2287","DOIUrl":null,"url":null,"abstract":"A sensitive quantification method for sufentanil in human milk was developed. Samples were prepared by liquid-phase extraction. Analytes were chromatographically separated. The mobile phase contained 10 mM ammonium formate (A) and methanol (B). The ratios of A to B were 50:50, 10:90,\n and 50:50 sequentially. For LC-MS-MS detection, transition of the protonated precursor ions to product ions (sufentanil: m/z 387.2→m/z 238.2; internal control fentanil: m/z 337.2→m/z 158.2) was monitored. The linear quantification range of sufentanil concentration was 3.2–400\n pg ·mL−1. The correlation coefficient (r2) was 0.996. The % RSD was less than 6.0% for intraday and less than 8.68% for interday precision. By this method, sufentanil contents were determined to be very low in the postnatal maternal milk samples.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biobased Materials and Bioenergy","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbmb.2023.2287","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A sensitive quantification method for sufentanil in human milk was developed. Samples were prepared by liquid-phase extraction. Analytes were chromatographically separated. The mobile phase contained 10 mM ammonium formate (A) and methanol (B). The ratios of A to B were 50:50, 10:90,
and 50:50 sequentially. For LC-MS-MS detection, transition of the protonated precursor ions to product ions (sufentanil: m/z 387.2→m/z 238.2; internal control fentanil: m/z 337.2→m/z 158.2) was monitored. The linear quantification range of sufentanil concentration was 3.2–400
pg ·mL−1. The correlation coefficient (r2) was 0.996. The % RSD was less than 6.0% for intraday and less than 8.68% for interday precision. By this method, sufentanil contents were determined to be very low in the postnatal maternal milk samples.