Optimization of process conditions for the development of pectin and glycerol based edible films: Statistical design of experiments

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Electronic Journal of Biotechnology Pub Date : 2022-01-01 DOI:10.1016/j.ejbt.2021.11.004

Shumyla Mehraj, Yamini Sudha Sistla

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引用次数: 8

Abstract

Background

Biopolymer based edible films have emerged as potential alternatives for conventional plastics in food packaging industry. The properties such as thickness, water vapour transmission rate (WVTR) and transparency of these films would be significantly influenced by the solution components and concentration and process conditions (pH, temperature and relative humidity of drying).

Results

Control and glycerol blended pectin films were developed as per 2³ (two-level three-factor) factorial design of experiments by varying glycerol fraction (25% and 40% w/w) and solution concentration (3% and 5% w/v). The films made from 5% solution showed good moisture barrier properties. Glycerol addition reduced the moisture barrier capability of the films compared to control pectin films. Statistical analysis suggests that, the solution pH and drying temperature considerably affect film properties while the effect of relative humidity of drying is not evident enough. However, the interaction effect of relative humidity (H) with the pH and temperature appeared significant. Regression models were fitted to the data by considering the main and interaction effects, which were significantly affecting a particular property.

Conclusions

Detailed analysis reveals that for obtaining pectin based films with less thickness, low WVTR and high transparency, the optimal conditions preferred are low pH = 3, high T = 48°C and low to medium humidity of drying (H = 40–50%). The fitted regression models were statistically significant at 90% confidence level, pass Lack-of-fit analysis and are adequate to describe the effects of different factors on the targeted film properties.

How to cite: Mehraj S, Sistla YS. Optimization of process conditions for the development of pectin and glycerol based edible films: statistical design of experiments. Electron J Biotechnol 2022;55. https://doi.org/10.1016/j.ejbt.2021.11.004

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以果胶和甘油为基础的可食性薄膜的工艺条件优化:实验统计设计

生物聚合物基可食用薄膜已成为食品包装工业中传统塑料的潜在替代品。溶液组分、浓度和工艺条件(pH、干燥温度和相对湿度)对膜的厚度、水蒸气透过率(WVTR)和透明度等性能有显著影响。结果通过不同的甘油分数(25%和40% w/w)和溶液浓度(3%和5% w/v)，采用23(二水平三因素)因子设计，制备了对照和甘油混合果胶薄膜。5%溶液制备的薄膜具有良好的防潮性能。与对照果胶膜相比，添加甘油降低了膜的防潮性能。统计分析表明，溶液pH值和干燥温度对薄膜性能影响较大，而干燥相对湿度对薄膜性能的影响不够明显。相对湿度(H)与pH、温度的交互作用显著。考虑到主效应和交互效应，对数据进行回归模型拟合，因为主效应和交互效应对某一特定属性有显著影响。结论为获得厚度小、WVTR低、透明度高的果胶基薄膜，最佳条件为低pH = 3、高T = 48°C、低至中等干燥湿度(H = 40-50%)。拟合的回归模型在90%的置信水平上具有统计学显著性，通过了缺乏拟合分析，足以描述不同因素对目标薄膜性能的影响。引文出处:Mehraj S, Sistla YS。开发果胶和甘油基食用薄膜的工艺条件优化:实验统计设计。中国生物医学工程学报(英文版);2009;16。https://doi.org/10.1016/j.ejbt.2021.11.004

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来源期刊

Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering