Prevalence of bovine herpes virus type 1 in small herds of young beef cattle in south-eastern Poland – a preliminary study

A. Wernicki, R. Urban-Chmiel, J. Rola, W. Socha, D. Stegierska, M. Dec, A. Puchalski
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引用次数: 2

Abstract

Abstract The study was performed on nasal swabs, tracheal samples, and sera obtained from young beef heifers aged between 6 and 12 months, from farms in eastern and south-eastern Poland. The samples were evaluated using bovine herpesvirus 1 (BHV-1) ELISA kits (ELISA BHV1 antibody and ELISA BHV1 antigen) and PCR. Among all the animals examined, 37 (32.2%) were positive in the ELISA BHV1 antigen test. The presence of BHV-1 was confirmed by PCR in 42 (36.5%) animals. In the ELISA BHV1 antibody test, 39 (33.9%) seropositive animals were identified. The presence of BHV-1 positive samples was observed in all the examined breeds of young cattle. There were no significant differences (P ≤ 0.05) in BHV-1 positive samples. The results indicate that the incidence of BHV-1 infections in feedlot cattle herds studied was 32.2%-36.5%, which suggests that preventive measures should be implemented in order to limit transmission of the virus.
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波兰东南部小群年轻肉牛中1型牛疱疹病毒流行率的初步研究
该研究对波兰东部和东南部农场6至12个月的小牛肉小牛的鼻拭子、气管样本和血清进行了研究。采用牛疱疹病毒1型(BHV-1)酶联免疫吸附测定试剂盒(ELISA BHV1抗体和ELISA BHV1抗原)和聚合酶链反应(PCR)对样品进行鉴定。ELISA BHV1抗原检测阳性37只(32.2%)。42只动物(36.5%)经PCR证实存在BHV-1。ELISA检测BHV1抗体阳性39只(33.9%)。在所检查的所有小牛品种中均观察到BHV-1阳性样本的存在。BHV-1阳性标本间差异无统计学意义(P≤0.05)。结果表明,养牛场牛群BHV-1感染率为32.2% ~ 36.5%,应采取预防措施,限制病毒的传播。
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