Experimental infection with epizootic haematopoietic necrosis virus (EHNV) of rainbow trout (Oncorhynchus mykiss Walbaum) and European perch (Perca fluviatilis L.)

E. Borzym, J. Maj-Paluch
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引用次数: 2

Abstract

Abstract The aim of this study was the determination of the susceptibility of Polish farmed redfin perch (Perca fluviatilis L.) and rainbow trout (Oncorhynchus mykiss Walbaum) to experimental infection with haematopoietic necrosis virus (EHNV). A bath challenge model was tested at two temperature ranges: 13-15°C and 20-22°C. After 7 d, the first clinical signs and mortality were observed in fish kept at these temperatures. Significantly more mortality cases were reported in the redfin perch population, reaching a maximum of 24% compared with 12% in the rainbow trout group at 20-22°C. EHNV was reisolated from redfin perch and rainbow trout tissue in cell culture and the infection was confirmed by a molecular method and histopathology during the duration of the experiment. This study revealed that fish from Polish farms can be susceptible to EHNV even at lower temperatures.
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虹鳟(Oncorhynchus mykiss Walbaum)和欧洲鲈鱼(pera fluviatilis L.)感染动物流行性造血坏死病毒(EHNV)的实验研究
摘要本研究旨在测定波兰养殖红鳍鲈(pera fluviatilis L.)和虹鳟鱼(Oncorhynchus mykiss Walbaum)对实验性感染造血坏死病毒(EHNV)的敏感性。在13-15°C和20-22°C两个温度范围内测试了浴池挑战模型。7 d后,在此温度下观察鱼的首次临床症状和死亡。据报道,在20-22°C的温度下,红鳍鲈种群的死亡率明显更高,最高可达24%,而虹鳟鱼组的死亡率为12%。在细胞培养中从红鳍鱼和虹鳟鱼组织中分离出EHNV,并在实验期间通过分子方法和组织病理学证实感染。这项研究表明,波兰养殖场的鱼即使在较低的温度下也容易感染EHNV。
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