Antioxidative potential of chrysin, a flavone in streptozotocin–nicotinamide-induced diabetic rats

T.A. Anitha , M. Rajadurai
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引用次数: 18

Abstract

Increasing evidence in both experimental and clinical studies suggests that oxidative stress has been suggested as a contributory factor in development and complications of both types of diabetes mellitus. The objective of the present study was to evaluate the protective effect of chrysin (5,7-dihydroxyflavone) against streptozotocin–nicotinamide (STZ–NA)-induced oxidative stress in male Wistar rats. Diabetes was induced by a single intraperitoneal (i.p.) injection of STZ (45 mg/kg body weight (b.w.)) dissolved in 0.1 mol/L citrate buffer (pH 4.5), 15 min after the i.p. administration of NA (110 mg/kg b.w.). The rats were divided into following groups: group 1: non-diabetic control, group 2: non-diabetic with chrysin (100 mg/kg b.w.), group 3: diabetic control, groups 4, 5 and 6 received chrysin as 25, 50, 100 mg/kg b.w., respectively. The oxidative stress was measured by examining the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and the non-enzymatic antioxidants, such as vitamin C, vitamin E and reduced glutathione (GSH) in liver and kidney. They were decreased while increasing the levels of LPO markers were observed in liver and kidney tissues of diabetic control rats as compared to normal control rats. Oral administration of chrysin (100 mg/kg/day) for 45 days caused a significant increase in the activities of both enzymatic and non-enzymatic antioxidants when compared to those of diabetic rats. These biochemical findings were also supported by histological studies on liver and kidney tissues. In conclusion, chrysin, especially at the dosage of 100 mg/kg b.w. can act as a potent antioxidant and anti-inflammatory agent in type II diabetic rats.

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黄酮类化合物大黄素在链脲佐菌素-烟酰胺诱导的糖尿病大鼠中的抗氧化潜力
实验和临床研究中越来越多的证据表明,氧化应激已被认为是两种类型糖尿病的发展和并发症的一个促进因素。本研究旨在探讨菊花素(5,7-二羟黄酮)对STZ-NA诱导的雄性Wistar大鼠氧化应激的保护作用。在给予NA (110 mg/kg b.w.) 15 min后,单次腹腔注射溶解于0.1 mol/L柠檬酸缓冲液(pH 4.5)中的STZ (45 mg/kg体重),诱导糖尿病。将大鼠分为3组:1组为非糖尿病对照组,2组为非糖尿病患者给予金菊素(100 mg/kg b.w), 3组为糖尿病对照组,4、5、6组分别给予金菊素25、50、100 mg/kg b.w。通过检测肝脏和肾脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽s -转移酶(GST)和非酶促抗氧化剂(维生素C、维生素E和还原性谷胱甘肽(GSH))的活性来检测氧化应激。与正常对照大鼠相比,糖尿病对照大鼠肝脏和肾脏组织中LPO标记物的水平升高,而LPO标记物的水平下降。与糖尿病大鼠相比,口服黄菊花素(100 mg/kg/天)45天可显著增加酶促和非酶促抗氧化剂的活性。这些生化结果也得到了肝脏和肾脏组织组织学研究的支持。综上所述,黄菊花素对2型糖尿病大鼠具有较强的抗氧化和抗炎作用,特别是在100 mg/kg b.w.剂量下。
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