Protective role of Solanum trilobatum (Solanaeace) against benzo(a)pyrene-induced lung carcinogenesis in Swiss albino mice

R. Venugopal, V. Mahesh, G. Ekambaram, A. Aadithya, D. Sakthisekaran
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引用次数: 2

Abstract

Objective

To investigate the protective effect of leaf extract of Solanum trilobatum (ELEST) against benzo(a)pyrene (BP) induced lung carcinogenesis.

Methods

Experiment was designed with the treatment regimen of ELEST [200 mg/kg body weight dissolved in dimethyl sulphoxide(DMSO)] for 4 weeks before (pre-initiation) and from 12th week after B(a)P (50 mg/kg body weight) induced lung carcinoma(post-initation).

Results

Administration of BP (50 mg/kg body weight) resulted in increased lipid peroxidation (LPO) and marker enzymes, such as arylhydrocarbon hydroxylase (AHH), gamma-glutamyl transpeptidase (γGT), 5′-nucleotidase (5′ND) and lactate dehydrogenase (LDH) along with decrease in the levels of tissue antioxidants, like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), vitamin E and vitamin C in mice. Significantly, ELEST modulated these alterations suggest the efficacy of ELEST in the chemotherapeutics of lung cancer. The histopathological studies also evidenced the protective efficiency of the extract against lung carcinogenesis. Further, significant increase in the levels of Cytochrome P450, Cytochrome b5, NADPH Cyt c reductase and decrease in UDP-glucuronyl transferase and quinone reductase was observed in microsomal fraction of lung and liver of BP-induced mice, whereas the treatment with ELEST resulted in reversal of modulations observed in the activities of detoxification enzymes.

Conclusions

Collectively, the present observations indicate that the treatment with ELEST exhibited protective and antioxidant effect against BP-induced lung carcinogenesis.

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三叶龙葵对苯并(a)芘致瑞士白化小鼠肺癌的保护作用
目的探讨三叶龙脑叶提取物(ELEST)对苯并(a)芘(BP)诱发肺癌的保护作用。方法在B(a)P (50 mg/kg体重)诱导肺癌(诱导后)前4周和12周采用ELEST [200 mg/kg体重溶解二甲基亚砜(DMSO)]治疗方案。结果BP (50 mg/kg体重)使小鼠脂质过氧化(LPO)和标记酶如芳烃羟化酶(AHH)、γ -谷氨酰转肽酶(γGT)、5′-核苷酸酶(5′nd)、乳酸脱氢酶(LDH)升高,组织抗氧化剂如超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、还原性谷胱甘肽(GSH)、维生素E和维生素C水平降低。值得注意的是,ELEST调节了这些改变,表明ELEST在肺癌化疗中的疗效。组织病理学研究也证实了该提取物对肺癌的保护作用。此外,bp诱导小鼠肺和肝脏微粒体部分细胞色素P450、细胞色素b5、NADPH Cyt c还原酶水平显著升高,udp -葡萄糖醛基转移酶和醌还原酶水平下降,而ELEST治疗导致解毒酶活性的调节逆转。综上所述,本研究结果表明,ELEST对bp诱导的肺癌具有保护和抗氧化作用。
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