Postoperative inflammatory reaction after endoscopic laser cyclodestruction in various modes (experimental study)

Abstract

The study and objectification of the degree of intensity of the postoperative inflammatory response, in our opinion, is of great importance after endoscopic laser cyclodestruction (ELCD), which is the most often performed in combination with cataract surgery, when it is usually about eyes with prospects for high visual functions. Features of morphological changes and to conduct an objective analysis of the severity of the postoperative inflammatory response after ELCD, accompanied by the POP-effect and without it, was the goal of the study. The experiment carried out on rabbits in two modes (on different eyes of each animal). In the first variant, surgical intervention performed to visual response obtained in the form of coagulation of processes with a power in the diapason from 0.3 to 0.5 W. In the second variant, until the visual realization of the biological vaporization effect in the diapason from 0.9 to 1.2 W. OCT of the anterior segment with prevailing coverage of the anterior chamber in 1, 7 and 14 days. The resulting frames standardized in size. They analyzed in the programm ImageJ. Series of semithin sections were made on an Ultracut E ultramicrotome (Reicher, Austria); photos were taken on an Axio A1 light microscope (Zaiss, Germany) with an AxioCam MRc5 camera (Zaiss, Germany). During the comparison of the groups, no statistically significant differences were found between them on the corresponding days of observation (p = 0.24). Light microscopy shows extensive damage to the epithelium of the processes with the migration of pigment granules outside the tissue, however the structure of the stroma remains ordered with the preservation of vascularization. Keywords: endoscopic laser cyclodestruction, postoperative inflammation, glaucoma