Method development and validation of RP-HPLC for simultaneous estimation of cilnidipine and valsartan in synthetic mixture

Abstract

Reverse phase-high performance liquid chromatography (RP-HPLC) method have been developed and validated for the estimation of Cilnidipine and Valsartan in bulk drug and synthetic mixture. The developed method is rapid, accurate, precise, simple and economical. The separation was carried out using Luna C18 100A◦ (250 mm×4.6 mm i.d.) 5 μm reverse phase column (phenomenex, luna®) in gradient mode, with mobile phase containing Acetonitrile: Water (85:15, v/v). The flow rate is 1.0 ml/min and effluents are monitored at 240 nm. Chromatogram showed peak at a retention time of 2.083 min for Cilnidipine and 5.458 min for Valsartan. The method is validated for system suitability, linearity, precision, accuracy specificity, ruggedness, robustness, LOD and LOQ. Recovery of Cilnidipine and Valsartan is found to be 100.36% and 100.14% respectively. The LOD and LOQ for estimation of Cilnidipine and Valsartan are found to be 0.037μg/ml, 0.31μg/ml and 0.206μg/ml, 0.62μg/ml respectively. Proposed method can be successfully applied for the quantitative determination of Cilnidipine and Valsartan in bulk drug and in synthetic mixture. © 2020 Published by Innovative Publication. This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/)