The detection of fowl adenovirus in chickens with hydropericardium syndrome in Isfahan and Charmahal-Va-Bakhtiyari provinces, Iran

Abstract

The Ascite and hydropericardium is most common in Isfahan and Chcharmahal-va-Bakhtiyari, Iran. For this, the study was achieved for investigation of AV role in induction of Ascite or hydropericardium in these regions. In this study, 20 broiler flocks suspected to Ascite and hydrpericardium were sampled from hydropericardium fluid. Then, DNA was extracted and amplified by specific primers. The amplified fragment for detection of AV was 896 bp. The results showed that 10 from 20 flocks, and 47 from 200 samples were positive to AV. The results revealed, the most of infected flocks were higher than 30 days old. In this study, the correlation between infectivity to AV and growing system were not significant. The infectivity rate in Isfahan and Chaharmahal-va-Bakhtiyari was not significant. Therefore, in addition to high altitude, AV infection can play a role in increasing the prevalence of hydropericardium syndrom in these areas.