{"title":"Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method","authors":"Nihan Kübra Belligün, B. Demir","doi":"10.5897/AJBR2019.1033","DOIUrl":null,"url":null,"abstract":"The single step three-phase partitioning (TPP) method was evaluated for the purification of invertase from Momordica charantia. Optimum conditions for TPP method, that is, 70% ammonium sulfate saturation, 1:0.5 t-butanol ratio and 30 min incubation time lead to 20.28% efficiency and 10.48 fold partial purification. Total protein decreased from 34.86 mg to 0.68 mg in the homogenate, and the specific activity of the enzyme increased from 21.67 to 227.02 U/mg protein. The purified enzyme showed maximum activity in 0.3 M pH 5.0 buffer at 50°C. In conclusion, the optimized single step TPP method revealed better purification. \n \n \n \n Key words: Three-phase partitioning (TPP), invertase, Momordica charantia, enzyme purification.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"39 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"African Journal of Biochemistry Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5897/AJBR2019.1033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
The single step three-phase partitioning (TPP) method was evaluated for the purification of invertase from Momordica charantia. Optimum conditions for TPP method, that is, 70% ammonium sulfate saturation, 1:0.5 t-butanol ratio and 30 min incubation time lead to 20.28% efficiency and 10.48 fold partial purification. Total protein decreased from 34.86 mg to 0.68 mg in the homogenate, and the specific activity of the enzyme increased from 21.67 to 227.02 U/mg protein. The purified enzyme showed maximum activity in 0.3 M pH 5.0 buffer at 50°C. In conclusion, the optimized single step TPP method revealed better purification.
Key words: Three-phase partitioning (TPP), invertase, Momordica charantia, enzyme purification.
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