Bio-hydrogen production by Pseudomonas stutzeri KF532951

International Journal of Chemical and Analytical Science Pub Date : 2013-12-01 DOI:10.1016/j.ijcas.2013.08.004

Sigamany Soniagandhi, Marimuthu Krishnaveni

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引用次数: 3

Abstract

Aim

Hydrogen production by biological means is more advantageous as it utilizes renewable sources and produces very low or no carbondioxide. Since, lignocellulosic feedstocks are cheaper source, the present study was aimed to isolate Pseudomonas stutzeri from soil sample at country bricks chamber furnace.

Methods

Final confirmation of the strain was done by 16S rRNA sequencing. The GC content was calculated. The isolated strain was inoculated in hydrogen production medium containing sugarcane bagasse replacing glucose. After the incubation period of 16 days, the gas collected was studied for hydrogen production through gas chromatography technique.

Results

According to sequencing and phylogenetic tree results, our isolate was found to be P. stutzeri KF532951. The GC content of our strain was above 53%. Our strain could produce up to 3.4 mol H₂/mol substrate by water displacement method.

Conclusion

On the basis of the above results, it is concluded that our strain is able to produce hydrogen biologically.

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stutzeri假单胞菌KF532951的生物制氢

生物制氢更有利，因为它利用可再生能源，产生非常低或不产生二氧化碳。由于木质纤维素原料是较便宜的原料来源，本研究旨在从乡村砖炉土壤样品中分离出斯图氏假单胞菌。方法采用16S rRNA测序法对菌株进行鉴定。计算GC含量。将分离菌株接种于含甘蔗渣代替葡萄糖的产氢培养基中。经过16天的孵育期，收集的气体通过气相色谱技术进行制氢研究。结果经测序和系统发育树分析，分离株为史都氏杆菌KF532951。本菌株的GC含量在53%以上。该菌株通过水置换法制备的底物H2/mol可达3.4 mol。结论该菌株具有生物产氢的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Chemical and Analytical Science

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