International Journal of Chemical and Analytical Science最新文献

Quantitative elemental analysis of Celocia argentea leaves by ICP-OES technique using various digestion methods 采用ICP-OES技术，采用多种消化方法对银角茶叶片进行定量元素分析

International Journal of Chemical and Analytical Science

Pub Date : 2013-12-01 DOI: 10.1016/j.ijcas.2013.08.003

Anil G. Markandeya , Narayan P. Firke , Shirish S. Pingale , Sunita Salunke-Gawali

Aim

Quantification of macronutrient, micronutrient and toxic elements from the leaves of Celocia argentea by inductively coupled plasma-optical emission spectrometry (ICP-OES) and to report efficient, reliable, eco friendly method of digestion for better recovery of the elements.

Methods

The five digestion methods are studied included, dry ashing method with HNO₃/HCl, wet digestion method with HNO₃/HClO₄ and HNO₃/H₂O₂, microwave digestion with HNO₃/HClO₄ and HNO₃/H₂O₂. ICP-OES operating parameter was optimized at radial and axial viewing along with the radio-frequency power, sample flush time and gas flow rate. The results of the elemental quantification were statistically analyzed using one way analysis of variance (ANOVA) by Kruskal–Wallis test.

Results

C. argentea exhibits good quantity of macronutrients like Mg, Ca S, P, K and Fe. The ashing and microwave digestion method were found to more reliable as compare to wet digestion method and hence recommended for better recovery. The element Ca, Cu and Fe gives better recovery by ash and microwave digestion method as compare to wet digestion method. Toxic elements show their concentration below permissible limit.

Conclusion

All the nutrients as well as toxic elements are quantified successfully by ICP-OES technique by all methods of digestion. Dry and wet digestion methods are tedious and time consuming compare to microwave digestion method, which is greener and simpler. Kruskal–Wallis test can be employed for non-parametric methods to compare more than two population average which do not require any specific form of the distribution of the population. The C. argentea can be used as supplementary food for deficiency of minerals like Fe, Mg and Ca.

目的用电感耦合等离子体发射光谱法(ICP-OES)定量测定青茶叶片中的常量营养素、微量营养素和有毒元素，并报道高效、可靠、环保的消化方法，以更好地回收这些元素。方法采用HNO3/HCl干灰化法、HNO3/HClO4和HNO3/H2O2湿消解法、HNO3/HClO4和HNO3/H2O2微波消解法研究5种消解方法。在径向和轴向观察下，结合射频功率、冲样时间和气体流量对ICP-OES工作参数进行优化。元素量化结果采用Kruskal-Wallis单因素方差分析(ANOVA)进行统计分析。青茶含有大量的镁、钙、硫、磷、钾和铁等常量营养素。灰化和微波消解法比湿消解法更可靠，回收率更高。与湿法消解法相比，灰分消解法和微波消解法对钙、铜、铁的回收率更高。有毒元素的浓度低于允许的限度。结论ICP-OES技术在各种消化方法下均能成功地定量出营养成分和有毒元素。干法和湿法消化与微波消化相比繁琐、耗时，更环保、更简单。Kruskal-Wallis检验可以用于非参数方法来比较两个以上的总体平均值，而不需要任何特定形式的总体分布。青茶可作为铁、镁、钙等矿物质缺乏的补充食品。

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引用次数: 13

AM1 and DFT: Conformational and vibrational spectra analysis of butyl methacrylate AM1和DFT:甲基丙烯酸丁酯的构象和振动光谱分析

International Journal of Chemical and Analytical Science

Pub Date : 2013-12-01 DOI: 10.1016/j.ijcas.2013.08.001

Ottman Belaidi , Tewfik Bouchaour , Ulrich Maschke

Aim

Butyl methacrylate is used with acrylic products in order to improve the final product according to the desired characteristics. In this work we are about to make an assignment of experimental infrared bands with the help of a theoretical quantum chemistry calculations. The exact knowledge of some bands which are not characteristics of acrylic materials will enable us to make a quick analysis with available techniques of low costs for mixtures of polymers based on acrylate and methacrylate molecules.

Materials and methods

In the experimental part, the infrared spectrum of butyl methacrylate is obtained by using an FTIR Perkin Elmer model 2000. In the computational part and as first step, the theoretical calculations are performed by the semi-empirical AM1 method for excluding similar structures of butyl methacrylate molecule by a meticulous conformational analysis. As a second step the obtained structures are optimized using DFT. The simulated frequencies are then scaled and a tentative assignment is made based on band intensities and PED percentages.

Results

The theoretical calculations predict the existence of five conformations two of them represent the majority of experimental bands in the infrared spectrum. The two less stable structures represent better the band at 546 cm⁻¹, while the assignment is very difficult for the band at 491 cm⁻¹.

将甲基丙烯酸丁酯与丙烯酸产品一起使用，以使最终产品达到所需的性能。在这项工作中，我们将在理论量子化学计算的帮助下，对实验红外波段进行分配。对一些非丙烯酸材料特征的条带的确切了解将使我们能够利用现有的低成本技术对丙烯酸酯和甲基丙烯酸酯分子聚合物混合物进行快速分析。材料与方法在实验部分，利用Perkin Elmer FTIR模型2000获得了甲基丙烯酸丁酯的红外光谱。在计算部分，作为理论计算的第一步，采用半经验AM1方法，通过细致的构象分析排除了甲基丙烯酸丁酯分子的相似结构。第二步，利用离散傅立叶变换对得到的结构进行优化。然后对模拟频率进行缩放，并根据频带强度和PED百分比进行暂定分配。结果理论计算预测了五种构象的存在，其中两种构象代表了红外光谱中大部分的实验波段。这两个不太稳定的结构在546 cm−1波段表现较好，而在491 cm−1波段则很难分配。

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引用次数: 6

Assessment of antioxidant potentiality of leafy herbs subjecting to different cooking temperatures 不同烹煮温度对叶类草本植物抗氧化能力的影响

International Journal of Chemical and Analytical Science

Pub Date : 2013-12-01 DOI: 10.1016/j.ijcas.2013.06.002

Besagarahalli Padmanabh Nandini, Mysore Shankar Singh Sudarshana, Nanjaiah Rajashekar

Aim

Leafy vegetables are known for high nutritional value and are being eaten raw or/subjected to various cooking methods. However, an attempt was made to evaluate antioxidant potentiality of leafy herbs viz., Rumex vesicarius L., Rumex acetosella L., Basella rubra L., and Basella alba L., subjecting to various cooking methods – to understand its property is stable or not, after subjecting to different cooking method.

Methods

For the first time antioxidant potentiality was evaluated at different cooking temperatures viz., 27 °C (Room temperature), 121 °C (Pressure cooker temperature), 100 °C (Micro wave oven temperature) with different concentration range (125–200 mg per 20 ml of methanolic extract) – by DPPH assay for selected plant extract.

Conclusion

R. acetosella L., and B. rubra L., (at room temperature) and all four plant extracts viz., R. vesicarius L., R. acetosella L., B. rubra L., and B. alba L. (at cooking temperature – 121 °C and micro oven – 100 °C) showed decreasing activity at 100, 125, and 150 mg/ml, and at 200 mg/ml, maximum (room temperature) antioxidant activity was observed. A better oxidative activity was observed at 200 μg/ml concentration at all temperatures for tested leafy vegetable, which can be attributed as dose dependant (200 mg/ml) for stable activity.

叶类蔬菜以其高营养价值而闻名，可以生吃或采用各种烹饪方法。然而，本研究试图对叶类草本植物——牛蒡子、牛蒡子、红Basella L.和白Basella L.在不同烹调方法下的抗氧化潜力进行评价，以了解其在不同烹调方法下的性能是否稳定。方法采用DPPH法，首次对所选植物提取物在27℃(室温)、121℃(高压锅温度)、100℃(微波炉温度)、浓度范围(125 ~ 200 mg / 20 ml)下的抗氧化能力进行了评价。在100、125和150 mg/ml时(室温)抗氧化活性最高，在200 mg/ml时(室温)抗氧化活性最高;在蒸煮温度- 121℃和微烤箱温度- 100℃时(室温)抗氧化活性最高;在200 μg/ml浓度下，叶类蔬菜在所有温度下均具有较好的氧化活性，其活性稳定与剂量有关(200 mg/ml)。

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引用次数: 4

Analysis of Copper, Zinc and Lead using Atomic Absorption Spectrophotometer in ground water of Jimma town of Southwestern Ethiopia 原子吸收分光光度法分析埃塞俄比亚西南部Jimma镇地下水中的铜、锌、铅

International Journal of Chemical and Analytical Science

Pub Date : 2013-12-01 DOI: 10.1016/j.ijcas.2013.07.006

Khalid Siraj, Shimeles Addisu Kitte

Aims

This study was design to determine the level of heavy metal contamination of ground water in Jimma town of Southwestern Ethiopia.

Methods

The sample had been collected from (6) different sites about (10) well and borehole water. Samples were analyzed using Atomic Absorption Spectrophotometer (AAS). Three heavy metals had been studied were Copper, Zinc and Lead. Their concentration and maximum contaminant levels (MCL) were compared with World Health Organization (WHO) data. The maximum contaminant levels according to WHO, for Copper, Zinc and Lead are 0.5, 3.0 and 0.01 mg/L respectively.

Conclusion

From the results obtained, none of the samples analyzed for Copper (0.025 mg/L) and Zinc (0.15 mg/L) concentration was found above the MCL but for Lead (0.02202 mg/L) concentration found above the MCL. However, the metals were present in 82.86% and 91.23% of the samples analyzed respectively. Almost 86.01% of the sample had detectable level of Lead. All the Lead concentration was above the MCL. In general, 86.70% of all samples analyzed contained one or more of three heavy metals. The results obtained from this study suggest significant risk to this population given for toxicity of these metals, well and borehole water are the only source of their water supply in this environment.

目的本研究旨在确定埃塞俄比亚西南部吉马镇地下水重金属污染水平。方法从6个不同地点采集井水和钻孔水样本。采用原子吸收分光光度计(AAS)对样品进行分析。对铜、锌、铅三种重金属进行了研究。它们的浓度和最大污染物水平(MCL)与世界卫生组织(WHO)的数据进行了比较。根据世界卫生组织的规定，铜、锌和铅的最大污染物水平分别为0.5、3.0和0.01毫克/升。结论铜(0.025 mg/L)和锌(0.15 mg/L)均未超过MCL，铅(0.02202 mg/L)均未超过MCL。然而，金属分别存在于82.86%和91.23%的分析样品中。86.01%的样品中铅含量可检出。所有铅浓度均高于MCL。总的来说，86.70%的分析样本含有一种或三种以上的重金属。从这项研究中获得的结果表明，鉴于这些金属的毒性，这一人群面临着重大风险，井水和钻孔水是他们在这种环境下供水的唯一来源。

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引用次数: 39

Bio-hydrogen production by Pseudomonas stutzeri KF532951 stutzeri假单胞菌KF532951的生物制氢

International Journal of Chemical and Analytical Science

Pub Date : 2013-12-01 DOI: 10.1016/j.ijcas.2013.08.004

Sigamany Soniagandhi, Marimuthu Krishnaveni

Aim

Hydrogen production by biological means is more advantageous as it utilizes renewable sources and produces very low or no carbondioxide. Since, lignocellulosic feedstocks are cheaper source, the present study was aimed to isolate Pseudomonas stutzeri from soil sample at country bricks chamber furnace.

Methods

Final confirmation of the strain was done by 16S rRNA sequencing. The GC content was calculated. The isolated strain was inoculated in hydrogen production medium containing sugarcane bagasse replacing glucose. After the incubation period of 16 days, the gas collected was studied for hydrogen production through gas chromatography technique.

Results

According to sequencing and phylogenetic tree results, our isolate was found to be P. stutzeri KF532951. The GC content of our strain was above 53%. Our strain could produce up to 3.4 mol H₂/mol substrate by water displacement method.

Conclusion

On the basis of the above results, it is concluded that our strain is able to produce hydrogen biologically.

生物制氢更有利，因为它利用可再生能源，产生非常低或不产生二氧化碳。由于木质纤维素原料是较便宜的原料来源，本研究旨在从乡村砖炉土壤样品中分离出斯图氏假单胞菌。方法采用16S rRNA测序法对菌株进行鉴定。计算GC含量。将分离菌株接种于含甘蔗渣代替葡萄糖的产氢培养基中。经过16天的孵育期，收集的气体通过气相色谱技术进行制氢研究。结果经测序和系统发育树分析，分离株为史都氏杆菌KF532951。本菌株的GC含量在53%以上。该菌株通过水置换法制备的底物H2/mol可达3.4 mol。结论该菌株具有生物产氢的能力。

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引用次数: 3

RP-HPLC method for simultaneous estimation of Rosuvastatin and Ezetimibe from their combination tablet dosage form 用反相高效液相色谱法同时测定瑞舒伐他汀和依折替米布联合片剂剂型的含量

International Journal of Chemical and Analytical Science

Pub Date : 2013-12-01 DOI: 10.1016/j.ijcas.2013.04.006

Mohammed Ishaq Beludari , Karanam Vanitha Prakash , Ghanta Krishna Mohan

Background

Rosuvastatin is an HMG Co-A inhibitor and Ezetimibe is an intestinal cholesterol absorption inhibitor. The combination formulation is used for the treatment of hypercholestrolemia. A simple, accurate and precise assay and rapid stability-indicating reversed-phase high performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the simultaneous estimation of Rosuvastatin (RSV) and Ezetimibe (EZE) from their combination drug product.

Methods

The proposed method is based on the separation of the two drugs in reversed-phase mode using Water’s C18 250 × 4.6 mm, 5μ column maintained at an ambient temperature. The optimum mobile phase consisted of Acetonitrile: water: 0.02 M phosphate buffer pH 8 (40:10:50 v/v), flow rate of mobile phase was set 1.0 mL min⁻¹ and PDA detection was performed at 230 nm. The method was validated according to ICH guidelines.

Results

It was found to be accurate and reproducible. Linearity was obtained in the concentration range of 30–90 μg mL⁻¹ for both RSV and EZE with correlation coefficients of 0.999 and 0.998 respectively. Mean percent recovery of triplicate samples at each level for both drugs were found in the range of 98% to 100% with RSD of less than 2.0%. Rosuvastatin, Ezetimibe and their combination drug product were exposed to thermal, photolytic, hydrolytic and oxidative stress conditions and the stressed samples were analyzed by the proposed method. There were no interfering peaks from excipients, impurities or degradation products due to variable stress conditions and the proposed method is specific for the simultaneous estimation of RSV and EZE in the presence of their degradation products.

Conclusion

The proposed method can be successfully applied in the quality control and stability samples of bulk manufacturing and pharmaceutical dosage forms.

舒伐他汀是一种HMG Co-A抑制剂，依折替米贝是一种肠道胆固醇吸收抑制剂。该联合制剂用于治疗高胆固醇血症。建立了一种简便、准确、精确、快速且具有稳定性指示的反相高效液相色谱(RP-HPLC)方法，并验证了同时测定瑞舒伐他汀(RSV)和依折替米贝(EZE)联合制剂中瑞舒伐他汀(RSV)和依折替米贝(EZE)的方法。方法采用水色谱柱C18 250 × 4.6 mm, 5μ，常温下反相分离两种药物。最佳流动相为乙腈:水:0.02 M磷酸盐缓冲液pH 8 (40:10:50 v/v)，流动相流速1.0 mL min - 1，在230 nm下进行PDA检测。根据ICH指南对方法进行了验证。结果该方法准确，重现性好。RSV和EZE在30 ~ 90 μg mL−1浓度范围内均呈线性关系，相关系数分别为0.999和0.998。两种药物在各水平上的平均回收率在98% ~ 100%之间，RSD < 2.0%。将瑞舒伐他汀、依折替米贝及其联合制剂分别置于热、光解、水解和氧化应激条件下，并采用所提出的方法对应激样品进行分析。由于不同的应力条件，没有来自辅料、杂质或降解产物的干扰峰，该方法适用于降解产物存在时RSV和EZE的同时估计。结论该方法可成功应用于原料药和制剂的质量控制和稳定性检测。

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引用次数: 37

Gas chromatography analysis for hydrogen production from Bacillus subtilis MKMP 2013 枯草芽孢杆菌MKMP 2013产氢气相色谱分析

International Journal of Chemical and Analytical Science

Pub Date : 2013-12-01 DOI: 10.1016/j.ijcas.2013.08.002

Chinnasamy Mohanapriya, Marimuthu Krishnaveni

Aim

Hydrogen is an uncontaminated energy carrier as it releases only water and no green house gas is released. Hence, bio-hydrogen production was aimed from Bacillus subtilis, a gram positive bacteria isolated near solid waste dumped place, Namakkal.

Methods

The isolated strain was confirmed by 16srRNA sequencing. Phylogenetic tree analysis was performed to know the nearest sequences. The isolated strain was allowed to grow in production medium containing beef, yeast extract, peptone, sodium chloride and sugarcane baggase for 16 days. Since, most of the hydrogen producing bacteria grow at broader pH, no pH control was opted during the study. After incubation period, the gas collected was analysed for hydrogen production by gas chromatography.

Results

The obtained sequence was submitted to genbank under the accession number KF484756. Phylogenetic analysis revealed the strain as B. subtilis. The hydrogen gas produced by B. subtilis using sugarcane bagasse was in the range of 1.3–3.3 mol H₂/mol substrate.

Conclusion

From this result, we can conclude that the isolated strain is able to produce hydrogen to certain extent and can be improved by gene modification.

氢是一种无污染的能源载体，因为它只释放水，不释放温室气体。因此，生物制氢的目标是利用枯草芽孢杆菌，一种革兰氏阳性细菌，分离于固体废物倾倒地纳玛卡尔附近。方法采用16srRNA测序法对分离菌株进行鉴定。进行系统发育树分析以确定最接近的序列。分离菌株在含有牛肉、酵母膏、蛋白胨、氯化钠和甘蔗袋酶的生产培养基中生长16天。由于大多数产氢细菌在较宽的pH下生长，因此在研究中没有选择pH控制。孵育期后，收集的气体用气相色谱法分析产氢。结果获得的序列已提交到基因库，登录号为KF484756。系统发育分析显示该菌株为枯草芽孢杆菌。枯草芽孢杆菌利用甘蔗渣产氢量为1.3 ~ 3.3 mol H2/mol底物。结论该菌株具有一定的产氢能力，并可通过基因修饰进行改良。

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引用次数: 3

Enhanced electrocapacitive performance of graphene oxide polypyrrole nanocomposites 增强氧化石墨烯聚吡咯纳米复合材料的电容性能

International Journal of Chemical and Analytical Science

Pub Date : 2013-09-01 DOI: 10.1016/j.ijcas.2013.09.001

Harish Mudila , Muhammad Ghulam Haider Zaidi , Sweta Rana , Varsha Joshi , Sarfaraz Alam

Aim

Graphene oxide/polypyrrole nanocomposites (PNCs) with enhanced electrocapacitive performance were prepared through a cationic surfactant assisted dilute solution polymerization of pyrrole in presence of GO at various concentrations (%, w/w) ranging 10.0–40.0 and ferric chloride as oxidant.

Methods

PNCs has been characterized through Fourier transformed infrared spectra, X-ray diffraction, scanning electron microscopy and simultaneous thermogravimetric-differential scanning caloriemetry. Electrochemical capacitance (C_s, F/g) of electrodes fabricated from PNCs over 316 stainless steel in presence of sulphonated polysulphone as binder has been investigated in KOH solution (1.0 M) with reference to Ag/AgCl at scan rate (V/s) ranging 0.2–0.001.

Results

PNCs with 20.0%, w/w of GO have shown highest C_s of 1073.64 as compared to 92.33 for pure PPy at a scan rate of 0.001 V/s. PNCs rendered a low electrocapacitive decrease during the first 500 cycles at scan rate of 0.1 V/s indicating their appreciable electrochemical performance for fabrication of supercapacitor electrodes.

摘要在氧化石墨烯浓度(%，w/w)为10.0 ~ 40.0的条件下，以氯化铁为氧化剂，采用阳离子表面活性剂辅助吡咯稀溶液聚合的方法制备了电容性能增强的氧化石墨烯/聚吡咯纳米复合材料(PNCs)。方法采用傅里叶变换红外光谱、x射线衍射、扫描电镜和热重-差示扫描量热法对spnc进行表征。以磺化聚砜为粘结剂，在KOH溶液(1.0 M)中，以Ag/AgCl为基准，在扫描速率(V/s)为0.2 ~ 0.001范围内，研究了pnc在316不锈钢上制备电极的电化学电容(Cs, F/g)。结果在扫描速率为0.001 V/s时，氧化石墨烯为20.0% w/w的spnc显示出最高的Cs值为1073.64，而纯PPy的Cs值为92.33。在扫描速率为0.1 V/s的前500个循环中，pnc呈现出较低的电容量下降，表明它们在制造超级电容器电极方面具有可观的电化学性能。

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引用次数: 5

Isolation and characterization of antimicrobial metabolite producing endophytic Phomopsis sp. from Ficus pumila Linn. (Moraceae) 榕树内生病原菌Phomopsis sp.抗菌代谢物的分离与鉴定。(桑科)

International Journal of Chemical and Analytical Science

Pub Date : 2013-09-01 DOI: 10.1016/j.ijcas.2013.08.006

Devaraju Rakshith , Parthasarathy Santosh , Sreedharamurthy Satish

Background/Aim

An endophytic fungus belongs to the genus Phomopsis was isolated from stem of Ficus pumila (Moraceae) and identified based on morphological and molecular characteristics.

Methods

Antimicrobial potential was investigated by agar plug, disc diffusion and TLC bioautography agar overlay methods. Detection of polyketide synthase (PKS) genes using DOP-PCR techniques.

Results

Endophytic Phomopsis sp. identified by morphological as well as molecular techniques, which exhibited broad a spectrum antimicrobial activity against Gram positive and Gram negative human and phytopathogenic bacteria and fungi. TLC bioautography detects the production of antimicrobial metabolites.

Conclusion

This preliminary study revealed that endophytic Phomopsis species are prolific producer of antimicrobial metabolites with wide range of antimicrobial activity.

背景/AimAn内生真菌属Phomopsis，从桑科无花果(Ficus pumila)茎中分离得到，并根据形态和分子特征对其进行了鉴定。方法采用琼脂塞法、圆盘扩散法和薄层生物自显影法测定微生物电位。聚酮合成酶(PKS)基因的dopp - pcr检测。结果经形态学和分子技术鉴定的内生磷藻对革兰氏阳性和革兰氏阴性的人、植物病原菌和真菌具有广谱抗菌活性。薄层色谱生物自显影检测抗菌代谢物的产生。结论本研究初步揭示了内生磷藻是一种多产的抗菌代谢物生产者，具有广泛的抗菌活性。

引用次数: 24

Antibacterial activity of pigment produced from Micrococcus luteus KF532949 黄体微球菌KF532949色素的抑菌活性研究

International Journal of Chemical and Analytical Science

Pub Date : 2013-09-01 DOI: 10.1016/j.ijcas.2013.08.008

Kaliappan Umadevi, Marimuthu Krishnaveni

Aim

The problems of synthetic pigment causing toxicity and carcinogenicity in the human body decrease its use. Therefore, interest in natural pigment production is increasing. Therefore, the marine environment has recently become an attractive research subject for many investigations, because of its rich biodiversity. Attempt has been initiated to isolate Micrococcus luteus from marina beach water.

Methods

The sea water sample isolated was allowed to grow in a Zobell marine agar medium and incubated at 37 °C for one week. The isolated strain from marine source was confirmed by 16S rRNA sequencing. The GC content was found to be 58.6%. Phylogenetic tree analysis was performed to know the sequences that are closely related. The strain was assessed for pigment production in Zobell marine broth and incubated at 37 °C for two days. The swab sticks collected from hospital were streaked directly on the labelled agar plates such as eosin methylene blue, MacConkey, nutrient, blood, mannitol salt agar and incubated at 37 °C for 24 h and characterized. The antibacterial activity of crude pigment was tested against wound isolates.

Results

The individual yellow colour colonies obtained was sequenced, submitted to gene bank under the accession number KF532949. Phylogenetic analysis revealed, that the identified strain M. luteus is closely related to various Micrococcus sp. The crude pigment showed promising results against Staphylococcus sp., Klebsiella sp., Pseudomonas sp. isolated from wound.

Conclusion

From this result, we can conclude that the isolated strain M. luteus is able to act against both gram positive and gram negative bacteria.

目的针对合成色素对人体的毒性和致癌性问题，减少其使用。因此，对天然色素生产的兴趣正在增加。因此，海洋环境由于其丰富的生物多样性，近年来成为许多研究的一个有吸引力的研究课题。已开始尝试从码头海滩水中分离黄体微球菌。方法分离的海水样品在佐贝尔海洋琼脂培养基中生长，37℃孵育1周。经16S rRNA测序证实该分离株来自海洋。GC含量为58.6%。通过系统发育树分析，确定了亲缘关系较近的序列。在Zobell海洋肉汤中评估菌株的色素产量，并在37℃孵育2天。将医院采集的拭子棒直接在标记好的伊红亚甲基蓝、麦康基、营养物、血液、甘露醇盐琼脂等琼脂板上划线，37℃孵育24 h并进行表征。研究了粗色素对伤口分离菌的抑菌活性。结果获得的单个黄色菌落测序成功，提交基因库，登录号为KF532949。系统发育分析表明，该菌株与多种微球菌有密切的亲缘关系，其粗色素对伤口分离的葡萄球菌、克雷伯氏菌、假单胞菌均有较好的抗菌效果。结论该分离菌株对革兰氏阳性菌和革兰氏阴性菌均有拮抗作用。

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引用次数: 39