Genetic variability studies in seven accessions of cowpea (Vigna unguiculata L. Moench.) using nine quantitative traits and chlorophyll content of leaves

R. Komolafe, J. Agbolade, A. Ajiboye, Rofiat Temitope Mustapha
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Abstract

Seven accessions of Vigna unguiculata were evaluated for variation in phenotypic traits and chlorophyll content of the cowpea leaves. Each accession was planted in a plastic container in triplicates in a Completely Randomised Block Design and evaluated based on nine quantitative traits. The morphological characters such as plant height, number of leaves per plant, leaf area, peduncle length, number of pods per plant, terminal leaf length, terminal leaf width, number of main branches and number of nodes were determined at maturity. The leaf chlorophyll content was determined using spectrophotometer set at a wavelength of 634nm, 648nm and 470nm. Accession UAMO910556 has the highest plant height of 91.58cm, followed by accession IT97K- 499-35 with plant height of 50.33cm, while accession NGB0050 has the lowest plant height of 22.43cm. Accession T107K-297-13 has the highest peduncle length, number of main branches, number of nodes on main stem, number of leaves and highest number of pod which are significantly different from other accessions for the trait studied. Accession T107K-297-13 also has the highest content of chlorophylls a and b and highest total chlorophyll content. This shows that accession T107K-297-13 is the most diverse genotype with high quality of trait and so it could be selected for further breeding work.
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7份豇豆(Vigna unguiculata L. Moench.) 9个数量性状和叶片叶绿素含量的遗传变异研究
对7份豇豆材料叶片的表型性状和叶绿素含量进行了评价。采用完全随机区组设计,将每一株种植在一个塑料容器中,一组三次,并根据9个数量性状进行评估。测定植株成熟时的株高、单株叶数、叶面积、花序梗长度、单株荚果数、顶叶长、顶叶宽、主枝数、节数等形态特征。采用634nm、648nm和470nm波长的分光光度计测定叶片叶绿素含量。植株高度最高的是UAMO910556,植株高度为91.58cm,其次是IT97K- 499-35,植株高度为50.33cm,植株高度最低的是NGB0050,植株高度为22.43cm。菌株T107K-297-13的花序梗长度、主枝数、主茎节数、叶片数和荚果数均高于其他菌株。加入T107K-297-13叶绿素a和b含量最高,叶绿素总含量最高。结果表明,加入T107K-297-13是品种多样性最高、性状质量较高的品种,可作为进一步选育工作的选择品种。
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