Hyperosmotic Stress Induced Actin Filaments Varying in Arabidopsis thaliana Suspension Cells

Lanchun Shi, Bochu Wang, Wei Gong, Yichuan Wang
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Abstract

With the suspension cells of Arabidopsis thaliana stably expressing the GFP-fimbrin1 fusion protein and confocal microscope, the actin filaments distribution of single cell was investigated in real-time during hyperosmotic exposure. Our study suggested that the maintenance and recovery of normal cell volume is related with actin cytoskeleton, and the reorganization of AFs is following the shrinkage of plasmalemma during plasmolytic process.
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高渗胁迫诱导拟南芥悬浮细胞中肌动蛋白丝的变化
利用稳定表达gfp - fibrin1融合蛋白的拟南芥悬浮细胞,在共聚焦显微镜下,实时观察高渗暴露过程中单细胞肌动蛋白丝的分布情况。我们的研究表明,正常细胞体积的维持和恢复与肌动蛋白细胞骨架有关,并且在质解过程中,AFs的重组是随着质膜的收缩而发生的。
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