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2009 3rd International Conference on Bioinformatics and Biomedical Engineering最新文献

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Effect of Asparagus saponins on HepG2 Apoptosis and Mitochondrial Membrane Potential and ROS Level 芦笋总皂苷对HepG2细胞凋亡及线粒体膜电位和ROS水平的影响
Pub Date : 2010-07-17 DOI: 10.1109/ESIAT.2010.5568423
Yubin Ji, C. Ji, L. Yue, L. Lang
To study the effect of Saponins of asparagus on HepG2 apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (Δψm) levels. Saponins of asparagus with different concentration was treated with HepG2 at different time, MTT assay was used to detect inhibitory rate, fluorescence staining was used to observe apoptosis morphology, flow cytometry was used to detect apoptosis rate and cell cycle, also ROS and Δψm were measured by flow cytometry. The results showed Saponins of asparagus inhibited cell proliferation, the IC50 on HepG2 was 172.3 mg/L. Apoptosis morphology was observed by fluorescence microscope. The cell cycle of HepG2 was arrested at S phase, G2/M phase percent decreased. After 72h the treated group appeared apoptosis peak, and apoptosis rate with high dose group 30.94 ± 1.74%; After 48h the ROS in treated group increased with high dose group 77.7 ± 4.5%; and Δψm decreased with high dose group 77.8 ± 1.9%. Saponins of asparagus can induce HepG2 cell apoptosis, which is caused by increasing ROS and decreasing Δψm of HepG2.
研究芦笋总皂苷对HepG2细胞凋亡、活性氧(ROS)和线粒体膜电位(Δψm)水平的影响。不同浓度的芦笋皂苷在不同时间用HepG2处理,MTT法检测抑制率,荧光染色观察细胞凋亡形态,流式细胞术检测细胞凋亡率和细胞周期,流式细胞术检测ROS和Δψm。结果表明,芦笋总皂苷对细胞增殖有抑制作用,对HepG2的IC50为172.3 mg/L。荧光显微镜下观察细胞凋亡形态。HepG2细胞周期停留在S期,G2/M期百分比下降。72h后,给药组出现凋亡高峰,高剂量组细胞凋亡率为30.94±1.74%;48h后,治疗组ROS较高剂量组升高77.7±4.5%;Δψm高剂量组降低77.8±1.9%。芦笋皂苷可诱导HepG2细胞凋亡,其机制是ROS升高,Δψm降低。
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引用次数: 0
Climate Warming Impact and Adaptation Activities of Rice Plantation in the Northeast China 气候变暖对东北地区水稻种植的影响及适应活动
Pub Date : 2009-07-14 DOI: 10.1109/ICBBE.2009.5162506
Y. Wang, Tan Xu
It is calculated that the average and standard deviation of the accumulate temperature from May to September for the period 1960-1999 of the Northeast China. The result shows that heat resource in almost all of the Northeast China increased. According to the quantitative relation between heat resource and rice yield per-unit-area of different rice varieties of earliest ripe, early ripe, medium ripe, medium-late ripe and late ripe, the model is established to calculate the expected rice yield per-unitarea using the method of expectation evaluation of risk-benefit decision-making. It is found climate warming induced positive effects on rice production in Heilongjiang and Jilin provinces, and induced negative effects in most areas of Liaoning Province. Keywordsadaptation; climate warming; expected yield perunit-area; Northeast China
计算了1960—1999年东北地区5 ~ 9月积温的平均值和标准差。结果表明,东北地区几乎全部地区的热量资源都有所增加。根据早熟、早熟、中熟、中晚熟和晚熟不同水稻品种的热量资源与单产的定量关系,采用风险效益决策的期望评价方法,建立了计算水稻单产的期望模型。结果表明,气候变暖对黑龙江、吉林两省的水稻生产有正向影响,对辽宁省大部分地区的水稻生产有负向影响。Keywordsadaptation;气候变暖;期望单产面积;中国东北
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引用次数: 1
Extended Iterative Nonlinear Regression Normalization for cDNA Gene Expression Data cDNA基因表达数据的扩展迭代非线性回归归一化
Pub Date : 2009-07-14 DOI: 10.1109/ICBBE.2009.5162838
Jianping Lu, Y. Wang
cDNA microarray expression data is widely used to help biomedical research. The data must be normalized because of various error functioned interferences existed. This paper has discussed the normalization for supervised multi-class (phenotype) data. All the classes are the type of multi-sample. Also, a reasonable hybrid cross-phenotype normalization (CPN) algorithm based on iterative nonlinear regression (INR) is proposed for this kind of array data set. As a part of this CPN algorithm, how to obtain a ldquobaselinerdquo from samples within a class by a statistical way and dynamic decision of reference/floating sample are discussed. Finally, experimental result is presented. The method in this paper has practical significance. Specifically, it can be used as a novel feature selection in gene pattern recognition.
cDNA微阵列表达数据广泛应用于生物医学研究。由于存在各种误差函数干扰,必须对数据进行归一化处理。本文讨论了有监督多类(表型)数据的归一化问题。所有的类都是多样本类型。针对这类阵列数据集,提出了一种基于迭代非线性回归(INR)的混合交叉表型归一化(CPN)算法。作为该CPN算法的一部分,讨论了如何通过统计方法从类内样本中获得最小基准值,以及参考/浮动样本的动态决策。最后给出了实验结果。本文的方法具有实际意义。具体来说,它可以作为一种新的特征选择方法用于基因模式识别。
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引用次数: 1
Inhibition Action on Xantine Oxidase by Biflavonoids from Selaginella labordei 卷柏生物黄酮对Xantine氧化酶的抑制作用
Pub Date : 2009-07-14 DOI: 10.1109/ICBBE.2009.5162968
Li Li, Ke-li Chen, Jia-Cheng Xu, Xiang Lei
Objective Xanthine oxidase inhibitory activities of the total biflavonoids and eight biflavonoid compounds isolated from the medicinal plants of Selaginella labordei have been investigated. Method The active compounds were screened out by measuring the inhibition rate and the 50% inhibitory concentration (IC 50) of each sample against xanthine oxidase (XOD) in vitro. Result Three kinds of biflavonoids were more active in inhibiting XOD than others, their inhibition rate was more than 85% at the concentration of 10 mugldrmL -1. IC 50 of the total biflavonoids, robustaflavone, 2",3"- dihydro -3',3"'- biapigenin and 2",3"-dihydroochnaflavone was 0.529, 0.162, 0.257, 0.254 mugldrmL -1 respectively. Conclusion The three kinds of biflavonoids were active compounds in inhibiting XOD from Selaginella labordei, and the total flavonoids was the main active position.
目的研究从药用植物卷柏(Selaginella labordei)中分离得到的总黄酮及8种化合物的黄嘌呤氧化酶抑制活性。方法通过测定各样品对黄嘌呤氧化酶(xanthine oxidase, XOD)的体外抑制率和50%抑制浓度(ic50)筛选出活性化合物。结果3种黄酮类化合物对XOD的抑制作用较强,在10 mugldrmL -1浓度下,其抑制率均大于85%。芦竹黄酮、2′,3′-二氢-3′、3′-双芹菜素和2′,3′-二氢萘黄酮的IC 50分别为0.529、0.162、0.257、0.254 mugldrmL -1。结论3种生物总黄酮均为抑制油布卷草XOD的活性化合物,其中总黄酮为主要活性部位。
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引用次数: 1
Expression of Caspase-3 Gene in Gastric Adenocarcinoma Cell Line SGC-7901 via Ad-FasL Caspase-3基因在胃腺癌细胞SGC-7901中的表达
Pub Date : 2009-07-14 DOI: 10.1109/ICBBE.2009.5162833
Shi-ying Zheng, J. Ge, Jun Zhao, D. Jiang, Hong Li
[Abstract] Objective To investigate the relationship between caspase-3 expression and apoptosis in the gastric adenocarcinoma cell line SGC-7901 transfected with the adenovirus-mediated FasL gene. Methods The human gastric adenocarcinoma cell line SGC-7901 was transfected with the adenovirus-mediated FasL gene. The expression of caspase-3 and apoptotic morphological changes were measured by flow cytometry, TUNEL, RT-PCR, and immunocytochemical assays. Results The expression of caspase-3 was low in the gastric adenocarcinoma cell line SGC-7901, but transfection of these cells with Ad-FasL enhanced the cellular expression of the caspase-3 gene and induced susceptibility to Fas/FasL-mediated apoptosis (25.4% vs. 7.2%, p < 0.001). Apoptosis exhibited a positive relationship with caspase-3 expression (γ = 0.647, p < 0.01) Conclusions The down-regulation of caspase-3 expression and resistance to Fas/FasL-mediated apoptosis in gastric adenocarcinoma cells were found to be related to the development and progression of the cells. FasL gene transfer may increase caspase-3 expression in gastric adenocarcinoma cells and enhance apoptosis in them. [Key words] membrane glycoproteins; caspases; gastric neoplasms/pathology; oncogenes; cell death
【摘要】目的探讨转染腺病毒介导FasL基因的胃腺癌细胞SGC-7901中caspase-3的表达与细胞凋亡的关系。方法用腺病毒介导的FasL基因转染人胃腺癌细胞SGC-7901。采用流式细胞术、TUNEL、RT-PCR和免疫细胞化学检测caspase-3的表达和凋亡形态学变化。结果在胃腺癌细胞SGC-7901中,caspase-3的表达水平较低,但转染Ad-FasL可增强caspase-3基因的表达,诱导Fas/ fasl介导的细胞凋亡易感性(25.4% vs. 7.2%, p < 0.001)。凋亡与caspase-3表达呈正相关(γ = 0.647, p < 0.01)结论caspase-3表达下调及对Fas/ fasl介导的凋亡的抵抗与胃腺癌细胞的发育进展有关。FasL基因转移可增加胃腺癌细胞中caspase-3的表达,促进细胞凋亡。【关键词】膜糖蛋白;还存在;胃肿瘤/病理学;致癌基因;细胞死亡
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引用次数: 2
Experiment Study of Fluoride Desorption with Groundwater Infiltration in Soil 地下水入渗对土壤中氟化物解吸的实验研究
Pub Date : 2009-07-14 DOI: 10.1109/ICBBE.2009.5162811
Zhongwei Zhao, Jian Zhao, Y. Zong
Fluoride contamination transport and adsorption study is a hot subject of soil environment research. Dynamic adsorption, desorption and kinetics study can help us deeply understand the migration and transformation mechanism of fluoride in water and soil system, and provide theoretical basis for environmental assessment and treatment. This paper reveals the fluoride desorption law with the infiltration of groundwater. It concluded that vertical distribution and desorption process of fluoride ions is different with content of clay and grain diameter. It is not a simple linear process for the decreasing concentration of fluoride. As in practical engineering application, we should make a special water import project for fluoride dilution according to the soil properties.
氟化物的迁移与吸附研究是土壤环境研究的热点。动态吸附、解吸和动力学研究可以帮助我们深入了解氟化物在水和土壤系统中的迁移转化机理,为环境评价和治理提供理论依据。本文揭示了地下水入渗对氟的解吸规律。结果表明,氟离子的垂直分布和解吸过程随黏土含量和粒径的不同而不同。氟浓度的下降不是一个简单的线性过程。在实际工程应用中,应根据土壤性质,制定专门的氟稀释引水方案。
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引用次数: 0
Estimation of Ultrasound Attenuation and its Application to Tissue Heterogeneity Study Using Nonlinear Least Square Data Fitting 基于非线性最小二乘数据拟合的超声衰减估计及其在组织异质性研究中的应用
Pub Date : 2009-07-14 DOI: 10.1109/ICBBE.2009.5162800
Xiaoying Li, Dong Liu
The aim of this paper is to provide a local attenuation method with applications to tissue heterogeneity estimation based on the peaks of ultrasound echo envelop data. To handle the frequency shift in the broadband ultrasound system, we use nonlinear least squares (NLS) data fitting directly to the local amplitude changes due to the tissue attenuation. By looking at the convergence of the NLS method, e.g., the Levenberg-Marquardt (LM) algorithm, we proposed a technique to test the tissue heterogeneity locally. System calibrations have been presented to compensate the aperture growth and beam diffraction effects. Algorithms have been verified both in phantom and in vivo images. Results showed that the proposed method can have 3.8% relative error in estimating attenuation coefficients of phantom images in a broadband system. It also showed the possible tissue heterogeneity from in vivo image.
本文的目的是提供一种局部衰减方法,并应用于基于超声回波包络数据峰值的组织非均匀性估计。为了处理宽带超声系统中的频移问题,我们使用非线性最小二乘(NLS)数据直接拟合由组织衰减引起的局部振幅变化。通过观察NLS方法的收敛性,例如Levenberg-Marquardt (LM)算法,我们提出了一种局部检测组织异质性的技术。提出了补偿孔径增长和光束衍射效应的系统校准方法。算法已经在幻影和活体图像中得到验证。结果表明,该方法在宽带系统中估计虚像衰减系数的相对误差为3.8%。从活体图像也可以看出可能存在的组织异质性。
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引用次数: 1
Sludge Stabilization of Vermifilter Andanalysis of Influence Factors 蚯蚓滤池污泥稳定性及影响因素分析
Pub Date : 2009-07-14 DOI: 10.1109/ICBBE.2009.5163405
Min Wu, Ning Liu, Chuanyin Wei, Meiyan Xing, Shanjie Lou
Under different operating modes, this paper studies on the sludge stabilization effect and of vermifilter in different medium, and analyzed efficiency factors of the sludge stability in filter. The results showed that after the treatment of vermifilter, the average sludge VSS/SS was smaller than 50%, the average VFA was 30-40%, and the average organism decomposition rate was up to 48-65%, so the system's whole effect was perfect. Filter medium was one of the most important factors which affect the function of sludge stabilization. Compared to silica sands, earthworms' sludge digestion efficiency in ceramic pellets was higher, and the system's whole effect was better. It was mostly due to the damage to earthworms caused by filter medium. The most suitable temperature for earthworms' sludge digestion efficiency was 23-28degC, and the whole effect was the best at the same condition. Since hydraulic overloading was somehow negative to earthworms' normal activities and its sludge digestion efficiency, it should be less than 6.00 m/d.
在不同的运行模式下,研究了不同介质中污泥的稳定效果,并分析了污泥在过滤器中稳定性的影响因素。结果表明,经过微滤池处理后,污泥VSS/SS平均小于50%,VFA平均为30-40%,生物分解率平均可达48-65%,系统整体效果较好。过滤介质是影响污泥稳定性能的重要因素之一。与硅砂相比,蚯蚓在陶瓷颗粒中的污泥消化效率更高,系统整体效果更好。主要原因是过滤介质对蚯蚓的伤害。蚯蚓污泥消化效率的最适温度为23 ~ 28℃,相同条件下整体效果最佳。由于水力超载对蚯蚓的正常活动和污泥消化效率有一定的负面影响,因此应小于6.00 m/d。
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引用次数: 0
A Novel Method Evaluating Vascular Resistance Based on Fourier Analysis for Pulse Waveform 基于脉冲波形傅里叶分析的血管阻力评估新方法
Pub Date : 2009-07-14 DOI: 10.1109/ICBBE.2009.5162219
Huifeng Yang, Qi Zhou, Jun Xiao
This paper proposed a novel method evaluating vascular resistance (VR). Its aim was for convenience and accuracy of non-invasive detection of VR. New index was defined through Fourier analysis for pulse waveform being representative of differently physiological and pathological status. The trial result showed a good correlation between this index and K value and this paper provided a new and doable suggestion for the clinically non-invasive detection of VR.
本文提出了一种评价血管阻力的新方法。其目的是为了方便和准确的无创检测虚拟现实。通过傅立叶分析,定义了能代表不同生理病理状态的脉搏波形的新指标。试验结果表明该指标与K值具有良好的相关性,为临床无创检测VR提供了新的可行建议。
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引用次数: 0
The Prediction of Pollution on Underground Water from Ash-Water of Power Plant Ash-Field 电厂灰场灰水对地下水污染的预测
Pub Date : 2009-07-14 DOI: 10.1109/ICBBE.2009.5163642
Peiyao Xu, Ya Zhang, Jiae Song, Zhirui Hao
On the basis of analysis of ash-water from Baoding power plant, chemical components of underground water and the condition of environmental hydrology the prediction on pollution from ash-water permeation was carried out by applying one- dimensional homogeneous mathematical model. The results show that ash-water will not take bad influence on the water quality of water source of Baoding city to ash-field 5000m in the short time. Keywords-ash-water; underground water pollution; pollution prediction At present, the domestic power plants in china use widely ash-hydraulic transport system, buck and fly ash are stored together in ash yard. Since most of ash yards belong to half closed circulating or half open system, there isn't ash yard becoming the center of underground water reverse filler. At the same time, the various contaminants in ash water seep with ash water to underground water causing pollution of underground water of ash yard around area. After contaminants enter underground water and move and spread in water, which will cause secure dangerous for the quality of water source for the resident living in the ash-field. Therefore the scientific prediction of pollution on underground water from ash-water is a very meaningful job for the power environmental protection workers. The ash yard of Baoding power plant is working object; we analyze the content of each contaminant in ash-water and underground water, and at the same time make detailed investigation of the environmental hydrogeology condition of ash yard around. On this foundation applying one-dimensional homogeneous mathematical model predict the moving condition in underground water of the contaminant Cl- and COD which is very important in ash-water in order to offering scientific basis for preventing pollution on underground water from ash-water.
在分析保定电厂灰水、地下水化学成分及环境水文条件的基础上,应用一维齐次数学模型对灰水渗透污染进行了预测。结果表明,在短时期内,含灰水对保定市水源至5000m灰田水质不会产生不良影响。Keywords-ash-water;地下水污染;目前,国内电厂普遍采用灰液输送系统,降压灰和飞灰在灰场同时贮存。由于灰场多为半封闭循环或半开式系统,因此灰场没有成为地下水反填料的中心。同时,灰水中的各种污染物随灰水渗入地下水,造成灰场周边地区地下水的污染。污染物进入地下水后在水中移动扩散,对生活在灰场的居民水源质量造成安全隐患。因此,科学预测灰水对地下水的污染对电力环保工作者来说是一项非常有意义的工作。以保定电厂灰场为工作对象;分析了灰水和地下水中各种污染物的含量,同时对灰场周围的环境水文地质条件进行了详细的调查。在此基础上,应用一维齐次数学模型预测了灰水中重要污染物Cl-和COD在地下水中的运移情况,为防治灰水中对地下水的污染提供科学依据。
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引用次数: 0
期刊
2009 3rd International Conference on Bioinformatics and Biomedical Engineering
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