New compound 6,7-dimethoxy-2-methylisoquinolinium from Indian medicinal plant Annona squamosa L.

Abstract

Objectives

Annona squamosa L. is a widely used Indian medicinal plant for the cure of deadly disease, diabetes. In recent decades, a great no. of chemical and pharmacological studies have been done on A. squamosa L. and still more research is required in order to search its bioactive components.

Methods

Bioassay dependent fractionation & isolation technique is used & repeated column chromatography was utilized to get pure compounds.

Results

Structure elucidation for compound A & compound B has been done using Mass, NMR, IR, UV, 1D & 2D NMR techniques.

Conclusion

In this paper we report one new compound from Indian medicinal plant A. squamosa L. which is characterized as 6,7-dimethoxy-2-methylisoquinolinium & is obtained from the twigs of the plant in its chloroform fraction. One known compound Rutin is also obtained from n-butanol fraction and is reported for the first time from this plant which is characterized as 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-{{(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyltetrahydro-2H-pyran-2-yloxy}methyl}tetrahydro-2H-pyran-2-yloxy]-4H-chromen-4-one.