Antitumor Activity of Escin in Mice Cervical Carcinoma U_(14) In Vivo and Human Cervical Carcinoma Hela Cell Line In Vitro

Abstract

Pollution made cancer a major disease threatening people's health. In the search for new cancer drug treatments, natural products perform an essential role. Escin, a mixture of triterpene saponins extracted from Aesculus Wilsonii Rehd., was used to analyze the cell growth inhibitory effects and the induction of apoptosis in human cervical carcinoma Hela cancer cell line in vitro. The results of MTT cell viability assay showed that escin could induce significant concentration-dependent and time-dependent inhibition of cell growth, and the cell cycle was arrested at G 1 /S phase in the escin-treated cells. Apoptosis were detected and assessed by Acridine Orange/Ethidium Bromide Staining and Flow Cytometry. The antitumor efficacy of escin on mice cervical carcinoma U_(14) in vivo was investigated, at dose of 2.8 mg/kg escin had rather high inhibition ratio(43.5%) on U_(14) tumor growth.