Anti Fungal Activity of Green Synthesized Copper Nanoparticles Using Plant Extract of Bryophyllum Pinnatum (Lam.) and Polyalthia Longifolia (Sonn.) R

Abstract

Plant-mediated nano-fabrication is a new area of nanotechnology that is preferred to traditional methods due to its advantages in terms of safety, cost efficiency, environmental friendliness, and biocompatibility. In the current study, copper chloride and copper sulphate are used as precursor materials to examine the effectiveness of green synthesized copper oxide nanoparticles using the plants Bryophyllum pinnatum (lam.) and Polyalthia longifolia (Sonn.) . Comparative study on the efficiency of the synthesized Copper oxide nanoparticles against each precursor has been studied. Different spectroscopic and microscopic characterization techniques such as UV- Visible spectrophotometer, X- ray powder diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM) were performed to confirm the presence of copper oxide nanoparticles. UV –vis spectrophotometer results confirmed the existence of copper oxide nanoparticles using Copper chloride and Copper sulphate precursor showed absorption at 235nm and 575nm respectively. X- Ray Diffraction results showed crystalline structure of the particles with three peaks at (111), (200) & (220) which confirmed the presence of copper oxide nanoparticle for both the precursors. FTIR results supported the existence of several functional groups involved in capping, reducing, and stabilizing copper oxide nanoparticles. The SEM image showed that the copper oxide nanoparticles were spherical in shape and ranged in size from 40 to 90 nm. Further, the Anti-fungal and Anti-bacterial activity of the synthesized nanoparticle for both the copper chloride and copper sulphate precursor were studied. The Study shown maximum zone of inhibition at 100µg/ml as 18mm and 25mm respectively against Galactomyces geotrichum. As a result of the high biological potentials and powerful Antifungal activity, the green synthesized copper oxide nanoparticles can be exploited in phytopathology to combat plant infections.