Background: Chronic kidney disease is a major public health issue which requires complex pharmacotherapy. This study was aimed to evaluate drug utilization pattern in chronic kidney disease patients. Method: A prospective observational study was conducted at Nephrology department in Rajiv Gandhi Cooperative Multispecialty hospital, Palakkad for a period of 6 month from July 2022 to January 2023. Medications were assessed by using WHO prescribing indicator and classified in the basis of Anatomic Therapeutic Classification. Results: A total of 120 patients were examined. According to the ATC categorization, out of a total of 921 medicines, cardiovascular drugs were most frequently administered. The average number of drugs per prescription was 7.6%. 11.3% of those medications were prescribed by their generic names. 49.9% of drugs were prescribed on the accordance with essential medicine list. The patient prescribed with an injection was 46% and patients prescribed with antibiotic were 25.5%. Poly pharmacy was executed in 86% of patients. Antihypertensive drugs were most frequently recommended class of drugs followed by hematopoietic drugs and vitamin and minerals in therapeutic wise classification of drugs. Conclusion: Of all drugs prescribed, cardiovascular drugs were commonly prescribed and prevalence of poly pharmacy is high in patients due to co morbidities.
{"title":"Evaluation of Drug Utilization Pattern in Patients with Chronic Kidney Disease","authors":"Sangeetha B, Thangamani S","doi":"10.13005/bbra/3157","DOIUrl":"https://doi.org/10.13005/bbra/3157","url":null,"abstract":"Background: Chronic kidney disease is a major public health issue which requires complex pharmacotherapy. This study was aimed to evaluate drug utilization pattern in chronic kidney disease patients. Method: A prospective observational study was conducted at Nephrology department in Rajiv Gandhi Cooperative Multispecialty hospital, Palakkad for a period of 6 month from July 2022 to January 2023. Medications were assessed by using WHO prescribing indicator and classified in the basis of Anatomic Therapeutic Classification. Results: A total of 120 patients were examined. According to the ATC categorization, out of a total of 921 medicines, cardiovascular drugs were most frequently administered. The average number of drugs per prescription was 7.6%. 11.3% of those medications were prescribed by their generic names. 49.9% of drugs were prescribed on the accordance with essential medicine list. The patient prescribed with an injection was 46% and patients prescribed with antibiotic were 25.5%. Poly pharmacy was executed in 86% of patients. Antihypertensive drugs were most frequently recommended class of drugs followed by hematopoietic drugs and vitamin and minerals in therapeutic wise classification of drugs. Conclusion: Of all drugs prescribed, cardiovascular drugs were commonly prescribed and prevalence of poly pharmacy is high in patients due to co morbidities.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135546248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tomato (Solanum lycopersicum L.) berries are in great demand across the globe for their nutritive and therapeutic properties. As agriculture land and fresh water resources are limited, the possibility of increasing the production of tomato is either by utilizing unproductive salt affected land for cultivation or unportable water high in salts for irrigation. Tomato is relatively susceptible to salinity during seed germination and seedling establishment phase. However, rapid and synchronized seed germination is essential for proper stand establishment in tomato for increasing its production in salinity stressed environments. Seed priming, a simple and lucrative approach for easing salt stress during the germination phase, is gaining popularity in tomato. Priming improves germination response and brings about certain biochemical changes that help primed tomato seeds to survive and grow under harsh conditions of salinity. This review discusses some of the seed priming methods such as hydropriming, osmopriming, solid matrix priming, hormonal priming, chemical priming, biopriming and physical priming that successfully mitigated the harmful effects of salt stress in tomatoes. Seed priming thus paves the way for utilization of saline land for growing tomato resulting in increased productivity and an improvement in tomato supply chain amidst rising demands.
{"title":"Seed Priming to Improve Tomato Productivity in Salinity Stressed Environments: A Review","authors":"Rupali Seth","doi":"10.13005/bbra/3133","DOIUrl":"https://doi.org/10.13005/bbra/3133","url":null,"abstract":"Tomato (Solanum lycopersicum L.) berries are in great demand across the globe for their nutritive and therapeutic properties. As agriculture land and fresh water resources are limited, the possibility of increasing the production of tomato is either by utilizing unproductive salt affected land for cultivation or unportable water high in salts for irrigation. Tomato is relatively susceptible to salinity during seed germination and seedling establishment phase. However, rapid and synchronized seed germination is essential for proper stand establishment in tomato for increasing its production in salinity stressed environments. Seed priming, a simple and lucrative approach for easing salt stress during the germination phase, is gaining popularity in tomato. Priming improves germination response and brings about certain biochemical changes that help primed tomato seeds to survive and grow under harsh conditions of salinity. This review discusses some of the seed priming methods such as hydropriming, osmopriming, solid matrix priming, hormonal priming, chemical priming, biopriming and physical priming that successfully mitigated the harmful effects of salt stress in tomatoes. Seed priming thus paves the way for utilization of saline land for growing tomato resulting in increased productivity and an improvement in tomato supply chain amidst rising demands.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135547130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study focused on the molecular phylogenetics for the identification of genus Chara species through 18S rRNA genes. Genus Chara sample is collected in the freshwater streams of Talakona region, Chittoor district, Andhra Pradesh and DNA was isolated. The 18S rRNA, gene sequence was used to carry out BLAST with the ‘nr’ database of NCBI GenBank database. First ten sequences were selected based on maximum identity score using multiple alignment software program Clustal W. Ribosomal Database Project was used for generating distance matrix and Molecular Evolutionary Genetics Analysis 6 Software was used for constructing the phylogenetic tree. Based on the maximum identifying score Chara species was identified as Chara foetida.
{"title":"18S rRNA Approach for Identification of Chara L. Species","authors":"Rekha Adimulam, P Sujathamma","doi":"10.13005/bbra/3154","DOIUrl":"https://doi.org/10.13005/bbra/3154","url":null,"abstract":"The study focused on the molecular phylogenetics for the identification of genus Chara species through 18S rRNA genes. Genus Chara sample is collected in the freshwater streams of Talakona region, Chittoor district, Andhra Pradesh and DNA was isolated. The 18S rRNA, gene sequence was used to carry out BLAST with the ‘nr’ database of NCBI GenBank database. First ten sequences were selected based on maximum identity score using multiple alignment software program Clustal W. Ribosomal Database Project was used for generating distance matrix and Molecular Evolutionary Genetics Analysis 6 Software was used for constructing the phylogenetic tree. Based on the maximum identifying score Chara species was identified as Chara foetida.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135546249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Using plant extracts in metal nanoparticle production is a straightforward, practical, cost-effective, and ecologically benign alternative to the use of harmful chemicals. As a consequence, several approaches for the quick manufacture of silver nanoparticles that are acceptable to the environment and use aqueous extracts of plant materials including leaves, bark, and roots have been published recently. In this paper, recent advancements in the area of environmentally friendly manufacturing of silver nanoparticles (AgNPs) using diverse plant extracts are highlighted and expanded upon, along with the potential use of these materials as antibacterial agents. A thorough analysis of the potential effects of phytochemical concentrations in plant extracts, extraction temperatures, extraction solvents, reaction temperatures, reaction times, reaction pHs, and precursor concentrations. Furthermore provided are extensive details on the potential mechanism by which AgNPs have strong antibacterial action and induce cell death in pathogens by interacting with their cell walls. In addition, whereas chemical methods for shape-controlled synthesis are widely established, controlling the shape of biologically generated AgNPs has several advantageous effects on its activities.
{"title":"A Recent Advancement Towards Herbal Biomass-Assisted Synthesis of Silver Nanoparticles","authors":"Ruchi Shivhare, Neelam Jain","doi":"10.13005/bbra/3134","DOIUrl":"https://doi.org/10.13005/bbra/3134","url":null,"abstract":"Using plant extracts in metal nanoparticle production is a straightforward, practical, cost-effective, and ecologically benign alternative to the use of harmful chemicals. As a consequence, several approaches for the quick manufacture of silver nanoparticles that are acceptable to the environment and use aqueous extracts of plant materials including leaves, bark, and roots have been published recently. In this paper, recent advancements in the area of environmentally friendly manufacturing of silver nanoparticles (AgNPs) using diverse plant extracts are highlighted and expanded upon, along with the potential use of these materials as antibacterial agents. A thorough analysis of the potential effects of phytochemical concentrations in plant extracts, extraction temperatures, extraction solvents, reaction temperatures, reaction times, reaction pHs, and precursor concentrations. Furthermore provided are extensive details on the potential mechanism by which AgNPs have strong antibacterial action and induce cell death in pathogens by interacting with their cell walls. In addition, whereas chemical methods for shape-controlled synthesis are widely established, controlling the shape of biologically generated AgNPs has several advantageous effects on its activities.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135546991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A central or peripheral somatosensory nervous system lesion or illness is a common cause of neuropathic pain. In this study, we address the most recent information on neuropathy, as well as the causes, symptoms, and treatments of neurodegenerative illnesses like Alzheimer's, Parkinson's, Huntington's, and Amyotrophic Lateral Sclerosis. While, in recent years, phenolic acid supplementation has been associated to enhanced cognitive function and the prevention of cognitive deterioration. The pharmacological effects of phenolic acid are discussed in this review. And gives the overview of role of Reactive oxygen Species (ROS), oxidative stress and antioxidants in neuropathy, and stated the strong relation between stress, tension, hectic lifestyle and neurodegenerative diseases.
{"title":"Neuroprotective Action of Polyphenols and Phenolic Compounds: An Overview","authors":"Shubham J. Khairnar, Ghanshyam B. Jadhav","doi":"10.13005/bbra/3132","DOIUrl":"https://doi.org/10.13005/bbra/3132","url":null,"abstract":"A central or peripheral somatosensory nervous system lesion or illness is a common cause of neuropathic pain. In this study, we address the most recent information on neuropathy, as well as the causes, symptoms, and treatments of neurodegenerative illnesses like Alzheimer's, Parkinson's, Huntington's, and Amyotrophic Lateral Sclerosis. While, in recent years, phenolic acid supplementation has been associated to enhanced cognitive function and the prevention of cognitive deterioration. The pharmacological effects of phenolic acid are discussed in this review. And gives the overview of role of Reactive oxygen Species (ROS), oxidative stress and antioxidants in neuropathy, and stated the strong relation between stress, tension, hectic lifestyle and neurodegenerative diseases.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135547132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mycobacterium tuberculosis, responsible for causing tuberculosis (TB) in humans, continues to pose a significant worldwide threat, causing extensive fatalities as the most prominent bacterial disease and urgent attention is required to develop novel anti-TB drugs. Throughout the history of medicine, natural remedies have consistently held a vital position, offering valuable references for the development of new drugs. The present study aimed to screen phytoconstituents of Derris indica as inhibitors of protein kinase B, an enzyme critical for cell wall synthesis of Mtb using in silico approach. Molecular docking of phytochemical library of D. indica against PknB was carried out to explore binding interactions, alongwith in silico toxicity prediction of the phytoconstituents. The shortlisted phytoconstituents demonstrated favorable pharmacokinetic characteristics suitable for oral absorption and met the criteria set by Lipinski's rule of five, indicating their potential as drug candidates. Six compounds (Pongaflavanol, Kaempferol, Quercetin, Karanjin, Ovaliflavanone A and Pongaglabrone) demonstrated significant binding interactions with the minimum binding energy ranging from -9.71 kcal/mol to -8.68 kcal/mol as compared with conventional synthetic drugs. These selected phytoconstituents may serve as valuable starting points for the future advancement of effective and safe antimycobacterial drug.
{"title":"Screening of Phytochemicals from Derris indica for Antimycobacterial Potential using Molecular Docking Analysis","authors":"Harsh Kashyap, Vanshika Makol, Manisha Khatri","doi":"10.13005/bbra/3142","DOIUrl":"https://doi.org/10.13005/bbra/3142","url":null,"abstract":"Mycobacterium tuberculosis, responsible for causing tuberculosis (TB) in humans, continues to pose a significant worldwide threat, causing extensive fatalities as the most prominent bacterial disease and urgent attention is required to develop novel anti-TB drugs. Throughout the history of medicine, natural remedies have consistently held a vital position, offering valuable references for the development of new drugs. The present study aimed to screen phytoconstituents of Derris indica as inhibitors of protein kinase B, an enzyme critical for cell wall synthesis of Mtb using in silico approach. Molecular docking of phytochemical library of D. indica against PknB was carried out to explore binding interactions, alongwith in silico toxicity prediction of the phytoconstituents. The shortlisted phytoconstituents demonstrated favorable pharmacokinetic characteristics suitable for oral absorption and met the criteria set by Lipinski's rule of five, indicating their potential as drug candidates. Six compounds (Pongaflavanol, Kaempferol, Quercetin, Karanjin, Ovaliflavanone A and Pongaglabrone) demonstrated significant binding interactions with the minimum binding energy ranging from -9.71 kcal/mol to -8.68 kcal/mol as compared with conventional synthetic drugs. These selected phytoconstituents may serve as valuable starting points for the future advancement of effective and safe antimycobacterial drug.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135546105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The North East of India is rich in biodiversity. There are various species of fish found in the rivers, lakes and ponds of the region. Different ethnic groups from this region use different types of techniques to catch these fish. To study different aspects of fish, the study of fishing gears is prerequisite. People of Assam use different types of indigenous fishing gears to catch fish. Most of these fishing gears are made of bamboo. In the current study, attempts were made to analyse the ichthyofaunal biodiversity along with indigenous tools used to catch fish in the wetlands areas nearby Nimati Ghat in Jorhat. A study was conducted to record the traditional fishing techniques used in these places, and it had been discovered that there were ten various kinds of traps, nets, and hooks used for fishing. Many synthetic nets replace the traditional fishing gears. During the study, different kinds of fish were recorded from the study areas.
{"title":"A Study on Ichthyofaunal Diversity and Fishing Gears Used in the Wetlands Areas Nearby Nimati Ghat, Jorhat, Assam","authors":"Gayotree Agni Borah, Somim Nasreen, Prasanna Kumar Dutta","doi":"10.13005/bbra/3152","DOIUrl":"https://doi.org/10.13005/bbra/3152","url":null,"abstract":"The North East of India is rich in biodiversity. There are various species of fish found in the rivers, lakes and ponds of the region. Different ethnic groups from this region use different types of techniques to catch these fish. To study different aspects of fish, the study of fishing gears is prerequisite. People of Assam use different types of indigenous fishing gears to catch fish. Most of these fishing gears are made of bamboo. In the current study, attempts were made to analyse the ichthyofaunal biodiversity along with indigenous tools used to catch fish in the wetlands areas nearby Nimati Ghat in Jorhat. A study was conducted to record the traditional fishing techniques used in these places, and it had been discovered that there were ten various kinds of traps, nets, and hooks used for fishing. Many synthetic nets replace the traditional fishing gears. During the study, different kinds of fish were recorded from the study areas.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135546846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The entire Alpan banana plant can be utilized medicinally. Since the banana's rapid global expansion, several non-wood plant fibers have been used as substitutes for wood pulp in the manufacture of pulp, paper, and paper board. Currently, modest commercial pulping operations use a range of non-wood fibers, including bamboo, jute, straw, rice, and abaca. The increasing number of banana varieties is a result of the everyday developments that are being achieved in several fields. The Materials and Methods: The purpose of this current study is to check fungal pathogenicity in infected Alpan banana fruit, molecular characterization of the pathogenic species and evaluation of biochemical activities in banana fruit. The studied biochemical activities include protein content, ascorbic acid, total sugar and total phenolic compounds. Ascorbic acid oxidase (AAO) and Phenol Oxidase (PPO) are also studied in the selected banana variety. Result: The fungal pathogenicity assay showed incidence of Aspergillus species on Alpan banana fruit. A very high prevalence of Aspergillus flavus and Aspergillus fumigatus were observed in examined fruits. Species confirmation were based on molecular characterization method and its subsequent bioinformatic analysis. Proteins, total phenolic content and Keto acid were observed to be 223.01, 47.32 and 964.0 mg/g respectively whereas PPO and AAO showed 0.800 and 0.175 U/ml content in the fruit sample. Conclusion:The study concludes the prevalence of fungal infection in ripe bananas by Aspergillus species and changes observed at their biochemical constituents level post fungal infestation
{"title":"Bioanalytical Studies on Fungal Pathogens Originating from Spoiled Alpan Banana (Musa Paradisiaca)","authors":"Amrendra Kumar singh, Prakash Chandra Gupta","doi":"10.13005/bbra/3150","DOIUrl":"https://doi.org/10.13005/bbra/3150","url":null,"abstract":"Background: The entire Alpan banana plant can be utilized medicinally. Since the banana's rapid global expansion, several non-wood plant fibers have been used as substitutes for wood pulp in the manufacture of pulp, paper, and paper board. Currently, modest commercial pulping operations use a range of non-wood fibers, including bamboo, jute, straw, rice, and abaca. The increasing number of banana varieties is a result of the everyday developments that are being achieved in several fields. The Materials and Methods: The purpose of this current study is to check fungal pathogenicity in infected Alpan banana fruit, molecular characterization of the pathogenic species and evaluation of biochemical activities in banana fruit. The studied biochemical activities include protein content, ascorbic acid, total sugar and total phenolic compounds. Ascorbic acid oxidase (AAO) and Phenol Oxidase (PPO) are also studied in the selected banana variety. Result: The fungal pathogenicity assay showed incidence of Aspergillus species on Alpan banana fruit. A very high prevalence of Aspergillus flavus and Aspergillus fumigatus were observed in examined fruits. Species confirmation were based on molecular characterization method and its subsequent bioinformatic analysis. Proteins, total phenolic content and Keto acid were observed to be 223.01, 47.32 and 964.0 mg/g respectively whereas PPO and AAO showed 0.800 and 0.175 U/ml content in the fruit sample. Conclusion:The study concludes the prevalence of fungal infection in ripe bananas by Aspergillus species and changes observed at their biochemical constituents level post fungal infestation","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135546850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CDH, an enzyme produced by wood-decomposing fungi, has diverse applications in biosensor production, bioremediation, and biomedical industries. In this study, CDH from Schizophyllum commune BCC26414 was purified using ammonium sulfate precipitation, DEAE-cellulose chromatography, and Sephadex G-200 chromatography. The purification fold achieved was 65.81 with a specific activity of 1612.34 U/mg. The purity and molecular weight of CDH was confirmed using native and SDS PAGE. Optimal temperature and pH were found to be 30°C and 5, respectively. The purified CDH exhibited stability over a wide pH range (3.5 to 6.5) for 24 hrs and retained complete activity at 40°C, with reduced activity at 50°C when observed for 150 min. KCl, MgSO4, ZnSO4, and NiCl2 at a concentration of 5 mM enhanced CDH activity and HgCl2 and CuSO4 inhibited the enzyme activity. The kinetic constants, Km and Vmax of CDH for lactose were observed to be 125 mM and 13.26 U/ml, respectively. The purified CDH may be utilized commercially in various applications.
{"title":"Cellobiose Dehydrogenase from Schizophyllum commune Bcc26414: Purification and Characterization","authors":"Vinita Yadav, Sadhana Nighojkar","doi":"10.13005/bbra/3141","DOIUrl":"https://doi.org/10.13005/bbra/3141","url":null,"abstract":"CDH, an enzyme produced by wood-decomposing fungi, has diverse applications in biosensor production, bioremediation, and biomedical industries. In this study, CDH from Schizophyllum commune BCC26414 was purified using ammonium sulfate precipitation, DEAE-cellulose chromatography, and Sephadex G-200 chromatography. The purification fold achieved was 65.81 with a specific activity of 1612.34 U/mg. The purity and molecular weight of CDH was confirmed using native and SDS PAGE. Optimal temperature and pH were found to be 30°C and 5, respectively. The purified CDH exhibited stability over a wide pH range (3.5 to 6.5) for 24 hrs and retained complete activity at 40°C, with reduced activity at 50°C when observed for 150 min. KCl, MgSO4, ZnSO4, and NiCl2 at a concentration of 5 mM enhanced CDH activity and HgCl2 and CuSO4 inhibited the enzyme activity. The kinetic constants, Km and Vmax of CDH for lactose were observed to be 125 mM and 13.26 U/ml, respectively. The purified CDH may be utilized commercially in various applications.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135546994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The current study sought to identify and quantify the prevalence of various risk factors for nephrolithiasis in the population of Jammu region. In the present study, a total of 100 study participants (50 cases and 50 controls) were enrolled. A detailed health questionnaire covering different risk factors viz., socio-demographic, biochemical, clinical and lifestyle parameters were designed for the study. The results revealed a significant association between physical inactivity and risk of nephrolithiasis. On comparison of biochemical parameters between the male and female patients, it was observed that overall male patients have slightly higher mean values of biochemical factors. Water being an important factor for reducing the risk of kidney stone but the consumption of water is also low (less than 3 liters/day) in most of the patients.
{"title":"Identification and Assessment of Non-Genetic Factors Contributing to Nephrolithiasis (Kidney Stone) in the Jammu Region","authors":"Kanak Mahajan, Meenakshi Bhagat, Jessica Cherukuri, Rahul Gupta, Amrit Sudershan, Rakesh Kumar Panjaliya, Parvinder Kumar","doi":"10.13005/bbra/3140","DOIUrl":"https://doi.org/10.13005/bbra/3140","url":null,"abstract":"The current study sought to identify and quantify the prevalence of various risk factors for nephrolithiasis in the population of Jammu region. In the present study, a total of 100 study participants (50 cases and 50 controls) were enrolled. A detailed health questionnaire covering different risk factors viz., socio-demographic, biochemical, clinical and lifestyle parameters were designed for the study. The results revealed a significant association between physical inactivity and risk of nephrolithiasis. On comparison of biochemical parameters between the male and female patients, it was observed that overall male patients have slightly higher mean values of biochemical factors. Water being an important factor for reducing the risk of kidney stone but the consumption of water is also low (less than 3 liters/day) in most of the patients.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135546104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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