Detection of Biofilm Formation in Classical and Hypervirulent Klebsiella pneumoniae

Iman Mohammad Mahmood Alansary, Nadal A. Al-Saryi
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引用次数: 1

Abstract

Klebsiella pneumoniae has considered as a relevant healthcare-associated pathogen, its risk of infections is increasing in the presence of medical devices. K. pneumoniae is known for its ability to form biofilm on biotic and abiotic surfaces. Biofilm of K. pneumoniae assists in bacterial protection from host immune responses and antibiotics. Hypervirulent Klebsiella pneumoniae (hvKp) emerges as a new pathotype, which first appeared in Asian Pacific Rim but spread globally. Thus, this study aimed to investigate the ability of K. pneumoniae including hvKp and potential hvKp isolates to form biofilm. One hundred isolates of K. pneumoniae were collected from different hospitals in Baghdad city. These isolates were identified by phenotypic characterization on selective agar plates, biochemical tests, VITEK II, and molecular identification. Biofilm formation was tested in these isolates by two methods, congo red and Tissue Culture Plate method. In congo red method, 33% of the isolates were biofilm producer and (63%) can form biofilm by TCP method divided as: 14% strong, 15 moderate, 34% weak, and 37% non-biofilm producer. The hvKp and potential hvKp isolates showed a variable ability to form biofilm as classical K. pneumoniae.
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经典和高致病性肺炎克雷伯菌生物膜形成的检测
肺炎克雷伯菌被认为是一种与卫生保健相关的病原体,在医疗器械的存在下,其感染风险正在增加。肺炎克雷伯菌以其在生物和非生物表面形成生物膜的能力而闻名。肺炎克雷伯菌的生物膜有助于细菌免受宿主免疫反应和抗生素的侵害。高致病性肺炎克雷伯菌(hvKp)作为一种新的病原体出现,它最初出现在亚太地区,但在全球范围内传播。因此,本研究旨在探讨肺炎克雷伯菌包括hvKp和潜在hvKp分离株形成生物膜的能力。从巴格达市不同医院收集了100株肺炎克雷伯菌分离株。这些分离物通过选择琼脂板上的表型表征、生化试验、VITEK II和分子鉴定进行鉴定。采用刚果红法和组织培养平板法检测分离菌株的生物膜形成情况。在刚果红法中,33%的分离菌能形成生物膜,63%的分离菌能形成生物膜,按TCP法分为:强分离菌14%,中等分离菌15株,弱分离菌34%,不形成生物膜的分离菌37%。hvKp和潜在hvKp分离株表现出与典型肺炎克雷伯菌形成生物膜的不同能力。
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