Conservation of the species Sternbergia colchiciflora Waldst. et Kit. through in vitro culture

Abstract

Sternbergia colchicifl ora Waldst. et Kit. is a critically endangered species, included in the Red Book of the Republic of Moldova. This species fulfi lls a decorative role in natural ecosystems and represents a natural source of products in the medical industry. Research on the conservation of the Sternbergia colchicifl ora species through in vitro culture is being carried out for the fi rst time in our country. The purpose of this study is to asepticize the plant material of the Sternbergia colchicifl ora species and introduce it into in vitro culture for subsequent multiplication and obtaining a large number of bulbs. Bulbs and seeds served as initial plant material. Explants grown on three types of nutrient media supplemented with various concentrations of plant growth regulators. Immature bulbs produced a higher number of bulbils than bulb fragments on medium V2: MS=100% (with the components of the basic medium) and additional addition of phytohormones 6 – benzylaminopurine (BAP) of 0,5 mg/l and naphthylacetic acid (ANA) of 0,25mg/l. The bulbs were replanted on the same type of nutrient medium for 5-6 weeks, developing under the conditions of the growth room, then being transferred to vegetative pots with a mixture of: peat, sand, perlite in a ratio of 1:0.5: 0.5.