Purification and Structural Characterization of an Antimicrobial Compound, Lipoxazolidinone a Produced by a Lactobacillus Apis YMP3

D. Kumar, Palanisamy Venkatachalam
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Abstract

ABSTRACT: Strains of Vibrio cholerae are one among the most causative and serious disease causing human pathogenic agents, its infections are caused mostly by ingesting contaminated water and/or food. According to the recent estimates, between 1.3 and 4.0 million individuals are infected all around the world every year. The lactic acid bacteria are an important class of probiotics microbes have their ability to produce diversified bioactive compounds, hence this study focused on the identification of a promising antimicrobial agent from a Lactobacillus apis YMP3. This strain was cultured on MRS broth and the cell free supernatant was ethyl acetate extracted for the antimicrobial agent. The crude extract was further purified with C18 silica gel column chromatography and structurally characterized by FT-IR, NMR, GC and MS/MS spectrum. The chemistry of the compound was confirmed as Lipoxazolidinone A which has the IUPAC name of (2E)-5-butyl-2-[(E)-4-methyl-2-oxoundec-3-enylidene]-1,3-oxazolidin-4-one. This is the first report of Lipoxazolidinone A produced by a bacterium, L. apis YMP3 which was originally isolated from yoghurt. This finding expands the scope of identifying more promising bioactive compounds from probiotic Lactobacillus sp., further, this systematic procedure for purification of this antimicrobial agent stood as the baseline data for more elaborate therapeutic studies in future.
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api乳酸杆菌YMP3抗菌化合物脂酰唑烷酮a的纯化及结构表征
摘要:霍乱弧菌是人类致病菌中最具致病性和严重致病性的菌株之一,其感染主要通过摄入被污染的水和/或食物引起。根据最近的估计,全世界每年有130万至400万人受到感染。乳酸菌是一类重要的益生菌微生物,具有生产多种生物活性化合物的能力,因此本研究的重点是从api乳杆菌YMP3中鉴定一种有前景的抗菌药物。在MRS培养基上培养,提取无细胞上清液乙酸乙酯作为抑菌剂。采用C18硅胶柱层析对粗提物进行了进一步的纯化,并用FT-IR、NMR、GC和MS/MS对粗提物进行了结构表征。化学性质证实该化合物为lipoxazolidone A, IUPAC命名为(2E)-5-butyl-2-[(E)-4-甲基-2- oxoundec3 -enylidene]-1,3-oxazolidin-4-one。本文首次报道了由原从酸奶中分离得到的L. api YMP3细菌产生的Lipoxazolidinone A。这一发现扩大了从益生菌乳酸杆菌中鉴定更有前景的生物活性化合物的范围,此外,该抗菌药物的系统纯化过程为未来更详细的治疗研究提供了基础数据。
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