An invitro study on antidiabetic and antiradical activities of flavonoid rich fraction of rubia cordifolia

Abstract

The main goal of the work was to evaluate the in vitro antidiabetic and antiradical activities of flavonoid rich fraction of Rubia cordifolia. The plant extracts were studied for their effect on inhibition of glycosylation of haemoglobin and glucose transport across yeast cells. It was found that the percentage increase of the rate of uptake of glucose into yeast cells was linear in different glucose concentrations used. Flavonoid rich fraction of Rubia cordifolia showing the maximum increase in 20mM Glucose concentration. Glycosylated hemoglobin is measured primarily to identify the average plasma glucose concentration over prolonged periods of time. The flavonoid rich fraction of Rubia cordifolia plants inhibited the activities of α -amylase and α-glucosidase in a concentration dependent manner, with results being comparable to the positive control. The results of the work indicated that the selected plants possessed considerable in vitro antidiabetic and antiradical activity and further these effects need to be confirmed using in vivo models for its effective utilization as therapeutic agents.