Synthesis and Biological Evaluation of Pyrazolyl Isoxazolines as Antimicrobial Agents

Abstract

Pyrazolyl isoxazolines were synthesized by 1,3-dipolar cycloaddition of pyrazolyl nitrile oxide with various activated alkene such as acrylonitrile, methyl acrylate and vinyl acetate in the presence of iodobenzene diacetate in methanol containing a catalytic amount of TFA at room temperature with an aim to explore their effect on in vitro growth of microorganism causing microbial infection. Eleven compounds were tested in vitro for their antibacterial activity against two Gram-positive bacteria namely, Staphylococcus aureus , Bacillus subtilis and two Gram-negative bacteria namely, Escherichia coli and Pseudomonas aeruginosa . All the synthesized compounds were also tested for their inhibitory action against two strains of fungus.