Isolation and characterization of antimicrobial metabolite producing endophytic Phomopsis sp. from Ficus pumila Linn. (Moraceae)

International Journal of Chemical and Analytical Science Pub Date : 2013-09-01 DOI:10.1016/j.ijcas.2013.08.006

Devaraju Rakshith , Parthasarathy Santosh , Sreedharamurthy Satish

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引用次数: 24

Abstract

Background/Aim

An endophytic fungus belongs to the genus Phomopsis was isolated from stem of Ficus pumila (Moraceae) and identified based on morphological and molecular characteristics.

Methods

Antimicrobial potential was investigated by agar plug, disc diffusion and TLC bioautography agar overlay methods. Detection of polyketide synthase (PKS) genes using DOP-PCR techniques.

Results

Endophytic Phomopsis sp. identified by morphological as well as molecular techniques, which exhibited broad a spectrum antimicrobial activity against Gram positive and Gram negative human and phytopathogenic bacteria and fungi. TLC bioautography detects the production of antimicrobial metabolites.

Conclusion

This preliminary study revealed that endophytic Phomopsis species are prolific producer of antimicrobial metabolites with wide range of antimicrobial activity.

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榕树内生病原菌Phomopsis sp.抗菌代谢物的分离与鉴定。(桑科)

背景/AimAn内生真菌属Phomopsis，从桑科无花果(Ficus pumila)茎中分离得到，并根据形态和分子特征对其进行了鉴定。方法采用琼脂塞法、圆盘扩散法和薄层生物自显影法测定微生物电位。聚酮合成酶(PKS)基因的dopp - pcr检测。结果经形态学和分子技术鉴定的内生磷藻对革兰氏阳性和革兰氏阴性的人、植物病原菌和真菌具有广谱抗菌活性。薄层色谱生物自显影检测抗菌代谢物的产生。结论本研究初步揭示了内生磷藻是一种多产的抗菌代谢物生产者，具有广泛的抗菌活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Chemical and Analytical Science

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