{"title":"Spectrofluorometric determination of L-tryptophan after preconcentration using multi-walled carbon nanotubes","authors":"E. Zolfonoun","doi":"10.24200/AMECJ.V2.I01.43","DOIUrl":null,"url":null,"abstract":"A solid-phase extraction method based on multi-walled carbon nanotubes (MWCNTs) was applied for the preconcentration of L-tryptophan prior to its spectrofluorometric determination. Due to the high surface area of MWCNTs, satisfactory concentration factor and extraction recovery can be achieved with only 10 mg MWCNTs in 5 min. The effects of pH, sorbent amount, eluent type and time on the recovery of the analyte were investigated. Under the optimum conditions, the detection limit for L-tryptophan was 8.9 ng mL−1. The precision of the method, evaluated as the relative standard deviation obtained by analyzing of 10 replicates, was 2.6%. The practical applicability of the developed method was examined using wheat and barley samples.","PeriodicalId":7797,"journal":{"name":"Analytical Methods in Environmental Chemistry Journal","volume":"184 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods in Environmental Chemistry Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24200/AMECJ.V2.I01.43","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 11
Abstract
A solid-phase extraction method based on multi-walled carbon nanotubes (MWCNTs) was applied for the preconcentration of L-tryptophan prior to its spectrofluorometric determination. Due to the high surface area of MWCNTs, satisfactory concentration factor and extraction recovery can be achieved with only 10 mg MWCNTs in 5 min. The effects of pH, sorbent amount, eluent type and time on the recovery of the analyte were investigated. Under the optimum conditions, the detection limit for L-tryptophan was 8.9 ng mL−1. The precision of the method, evaluated as the relative standard deviation obtained by analyzing of 10 replicates, was 2.6%. The practical applicability of the developed method was examined using wheat and barley samples.