Cytotoxicity induction by ethanolic extract of Acalypha indica loaded casein-chitosan microparticles in human prostate cancer cell line in vitro

Biomedicine & Preventive Nutrition Pub Date : 2014-07-01 DOI:10.1016/j.bionut.2013.03.009

Kanchana Amarnath, Jeevitha Dhanabal, Isha Agarwal, Srividya Seshadry

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引用次数: 15

Abstract

The present exploration reports an extensive evaluation of in vitro anticancer efficacy of a novel aqueous ethanolic extract of Acalypha indica (ETAI) loaded chitosan-casein (CS-CT) microparticles in a cancer cell line model. Spherically shaped ETAI loaded CS-CT (CS/CT/ETAI) microparticles were prepared by colloidal coacervation technique in a completely aqueous environment with an entrapment efficiency and particle size of 85.30 ± 4.10 and 2 ± 0.96 μm respectively. Cytotoxicity, as investigated on human prostate cancer cell line (PC3) by MTT assay, revealed insignificant differences between free ETAI and CS/CT/ETAI microparticles treated cells in the first 24 h, while higher cytotoxicity was demonstrated by CS/CT/ETAI microparticles, following 72 h of incubation. Percentage of cytotoxicity also demonstrated by LDH assay, which shown the concentration dependent leakage of LDH from PC3 cells exposed to free ETAI and CS/CT/ETAI microparticles The use of significantly low concentration of Acalypha indica loaded with CS/CT is a much better approach in comparison to the use of free ETAI for cancer treatment in future.

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载酪蛋白壳聚糖微颗粒的白果乙醇提取物对体外人前列腺癌细胞的细胞毒性诱导作用

本研究报道了一种新型负载壳聚糖-酪蛋白(CS-CT)微粒的猕猴桃水乙醇提取物(ETAI)在癌细胞系模型中的体外抗癌效果的广泛评估。采用胶体凝聚技术在全水环境下制备了球形装载ETAI的CS-CT (CS/CT/ETAI)微粒子，其包封效率为85.30±4.10 μm，粒径为2±0.96 μm。MTT法研究了游离ETAI与CS/CT/ETAI微颗粒对人前列腺癌细胞(PC3)的细胞毒性，结果表明，游离ETAI与CS/CT/ETAI微颗粒在处理前24 h的细胞毒性差异不显著，而CS/CT/ETAI微颗粒在孵育72 h后表现出更高的细胞毒性。LDH实验也证明了细胞毒性的百分比，显示了暴露于游离ETAI和CS/CT/ETAI微粒的PC3细胞中LDH的浓度依赖性泄漏。与使用游离ETAI相比，使用低浓度的装载CS/CT的白果树是一种更好的癌症治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedicine & Preventive Nutrition

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