CONSERVATIVE TREATMENT ALTERNATIVES TO REMOVE STAINS DUE TO DENTAL FLUOROSIS TYPE IV. IN VITRO STUDY

Mabel Elizabeth Hernández Aguilera, Juan Alberto Hernández Castillo, Jimena Patricia Zamarripa Gonzalez, María Teresa Ley Fong, Sergio Eymard Trejo Tejeda, Mario Alberto Palomares Rodriguez
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Abstract

Introduction: Type IV dental fluorosis compromises the aesthetic and psychological expectations of patients. The main cause is excessive intake of fluoride during dental development. Conservative treatments for the removal of some pigmentations are microabrasion and combination with tooth whitening. There are new products on the market, such as Antivet®, which is proposed in this study for comparison with other treatments. Materials and methods: 12 dental organs were stored in 0.2% thymol at 37°C, which were divided into 4 groups. Group 1 (OP): (n=3) Opalustre®, group 2 (OP+PH): (n=3) treated with Opalustre® adding Whiteness HP Maxx 35%®, group 3 (ANT) (n=3): treated with Antivet® and group 4 (ANT+PH): (n=3) treated with Antivet® and Whiteness HP Maxx 35%®. Microabrasion was performed with Opalustre® for 60 seconds at 10 intervals for a total of 10 minutes of exposure. The abrasion with Antivet® was carried out for 10 minutes, at the end the neutralizing solution of the same treatment was placed for one minute. Whiteness HP Maxx 35%® was applied seven days after the treatments with Opalustre® and Antivet®, for 15 minutes, making 2 more applications for a total of 45 minutes of exposure. The specimens were taken to SEM/EDS (JEOL FE-SEM JSM-7800F) and color measurements were taken with VITA EasyShade V ® spectrophotometer. Statistical process control: The Kolmogórov-Smirnov test, analysis of variance (ANOVA) was performed with a significance level of .05 in the statistical package IBM SPSS Statistics 23. Results: in the ANT group, SEM images show the loss of the aprismatic layer and an etched surface in which the periphery of the enamel prisms appeard removed. On the ANT+PH group an etched surface was observed around the enamel rods, slight erosion and porosity on the enamels surface. In the mineral content there was a statistically significant difference between the study groups of the minerals C, P, F and Ca (p < 0.05) For the color measurement, there was a statistically significant difference in the ΔE value between the OP group compared to the ANT group with a p-value of 0.031, being the highest value in ANT. Conclusion: The Antivet® treatment showed a more significant favorable color change in the L*, a*, and b* values, as well as an increase in the concentration of Ca and P.
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iv型氟斑牙的保守治疗方法。体外研究
IV型氟斑牙损害了患者的审美和心理期望。主要原因是牙齿发育过程中摄入了过量的氟化物。对于一些色素的去除,保守的治疗方法是微磨牙和联合牙齿美白。市场上有一些新产品,如Antivet®,本研究提出将其与其他治疗方法进行比较。材料与方法:12个牙器官37℃保存于0.2%百里香酚中,分为4组。第1组(OP): (n=3) Opalustre®,第2组(OP+PH): (n=3)用Opalustre®加Whiteness HP Maxx 35%®处理,第3组(ANT) (n=3)用Antivet®处理,第4组(ANT+PH):(n=3)用Antivet®加Whiteness HP Maxx 35%®处理。使用Opalustre®进行微磨损,每隔10次,持续60秒,总共曝光10分钟。使用Antivet®进行10分钟的磨损,最后放置相同处理的中和液1分钟。在Opalustre®和Antivet®处理后7天,使用HP Maxx 35%®美白产品15分钟,再使用2次,总共45分钟。将样品进行SEM/EDS (JEOL FE-SEM JSM-7800F)扫描,用VITA EasyShade V®分光光度计进行颜色测量。统计过程控制:在IBM SPSS Statistics 23统计软件包中进行Kolmogórov-Smirnov检验,方差分析(ANOVA),显著性水平为0.05。结果:在ANT组中,扫描电镜图像显示牙釉质棱柱层缺失,表面蚀刻,牙釉质棱柱的外围似乎被移除。在ANT+PH组,牙釉质棒周围有蚀刻表面,牙釉质表面有轻微的侵蚀和孔隙。在矿物质含量上,C、P、F、Ca的研究组之间差异有统计学意义(P < 0.05)。在颜色测量上,OP组与ANT组的ΔE值差异有统计学意义,P值为0.031,是ANT中最高的值。结论:Antivet®处理对L*、a*、b*值的颜色变化更有利,Ca、P浓度升高。
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