A New Approach To Description Of Equilibrium State In The Extraction System Of Vegetal Raw Material-Extractant

Abstract

Aim of this study is the theoretical justification and experimental confirmation of new approach to description of equilibrium state in extraction system vegetal raw materialextractant. In framework of this study the milled raw material was used containing the particles sized 0,1-0,5 mm: roots and rhyzomes of Glycyrrhiza glabra or uralensis, leaves of Uvaurzi, leaves of Eucalyptus viminalis, flowers of Helichrysum arenarium, flowers of Calendula officinalis and etc. The process of equilibrium establishment was studied at temperature levels such as 4, 20, 40 and 60±1oС, wherein the method of simple maceration was implemented for 24 hours of decoction. Interphase distribution of BAS was studied using the proportion of raw material weight / extractant volume as 1:5, 1:10, 1:20, 1:40. The watery ethanol solution of 70 or 80±1 % vol. was chosen as an extractant for the purpose of this study. Assay test of BAS in raw material was carried out with the help of RP HPLC instrumentation method. Experimental points for all the studied BAS from various types of vegetal raw materials are well-approximated by regression lines built up in theoretically predicted coordinates, which is confirmed by the high value of determination coefficient R2≥0.99. The new approach has been proposed to description of equilibrium state in extraction system vegetal raw material-extractant, which is based on the promotion of hypothesis, according to which the mechanism of interphase molecular distribution of BAS in extraction system is explained and described by classic Boltzmann distribution for discrete values of molecular energy (or Fermi-Dirac quantum distribution). The developed and experimentally confirmed mathematical model was based on the promoted hypothesis, and it successfully described the relation of the main parameters of extraction system, which allows to develop the rational technology of tincture or extracts manufacturing. Keywords—extraction, vegetal raw material, biologically active substances, equilibrium, Boltzmann distribution for discrete values of molecular energy, Fermi-Dirac quantum distribution.