{"title":"Psicofuranine: Correlation of Assay Methods in Acid Degradation Studies*","authors":"Edward R. Garrett, Ladislav J. Hanka","doi":"10.1002/jps.3030490811","DOIUrl":null,"url":null,"abstract":"<div><p>The unnatural nucleoside, psicofuranine, is bimolecularly hydrolyzed by acid to adenine and the sugar psicose. Adenine appears to reverse the biological activity of psicofuranine in the plate-disk method against <em>S. aureus</em> so that standard curves must be prepared with the same amount of adenine as the material to be assayed. When this phenomenon is accounted for, the chemical and biological assays are shown to correlate. The kinetic constants for the acid catalyzed hydrolysis of the nucleoside have been determined.</p></div>","PeriodicalId":100839,"journal":{"name":"Journal of the American Pharmaceutical Association (Scientific ed.)","volume":"49 8","pages":"Pages 526-529"},"PeriodicalIF":0.0000,"publicationDate":"1960-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jps.3030490811","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Pharmaceutical Association (Scientific ed.)","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S009595531536265X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
The unnatural nucleoside, psicofuranine, is bimolecularly hydrolyzed by acid to adenine and the sugar psicose. Adenine appears to reverse the biological activity of psicofuranine in the plate-disk method against S. aureus so that standard curves must be prepared with the same amount of adenine as the material to be assayed. When this phenomenon is accounted for, the chemical and biological assays are shown to correlate. The kinetic constants for the acid catalyzed hydrolysis of the nucleoside have been determined.
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