Isolation and Identification of Antioxidant Compounds from Stained Pyrus serotina Fruit Extract for alternative tea development

Abstract

For alternative tea development, 2 kg of dried stained Pyrus serotina was extracted with 70 % MeOH aqueous solution, and the extract was solvent-fractionated with EtOAc, n-BuOH and Water. Two types of bioactive compounds were separated from the EtOAc fraction, by purification with silica gel and octadecyle silica gel (ODS) column chromatography. The chemical structure of the compounds were identified as quercetin (1) and geniposide (2) by analyzing spectral data with NMR, MS and IR. For the evaluation of antioxidant activity, DPPH inhibitory efficacy test and ROS production inhibitory efficacy test were performed. First, as a result of measuring the DPPH inhibitory efficacy of two samples up to the highest concentration of 50 ppm in the DPPH inhibition efficacy test, the IC50 values of NICS-1 and NICS-2 were measured to be 25.554 ppm and 22.943 ppm, respectively. In ROS production inhibitory efficacy test, the ROS production was reduced in a concentration-dependent manner in both samples, and statistically significantly inhibited ROS production compared to the UV irradiation group. According to recent reports, this study is expected to develop eco-friendly sustainable health drinks using pears and cheese and functional raw materials using pears and teeth through organic cosmetics.