Biocompatible Protein (IgG) Modified Up-conversion Nanoparticles (NaGdF4: Yb3+, Er3+) Deposited by Matrix Assisted Pulsed Laser Evaporation (MAPLE)

Abstract

In this study, up-conversion nanoparticles (NaGdF4: Yb3+, Er3+) with/without Immunoglobulin G (IgG) modification were produced by a one-pot synthesis method. The average size of the UCNPs is 50 ± 5 nm. Following that, the up-conversion nanoparticles with/without IgG modification were deposited on the culture dish with glass bottom Matrix assisted pulsed laser evaporation (MAPLE) technique. Nd:YAG laser (Ȝ = 532 nm, IJfwhm § 200μs, Ȟ = 10 Hz) was applied in this deposition. The antibody-modified nanoparticles deposited on the bottom of culture dish by MAPLE process are characterized by energy-dispersive X-ray spectroscopy (SEMEDS) and Fourier transform infrared spectroscopy (FT-IR). In addition, human umbilical vein endothelial cells (HUVECs) are cultured on the culture dish with the deposition of up-conversion nanoparticles with/without antibody, IgG. No toxic effect is imposed on cells. The results of this work indicate the deposition of antibody-modified nanoparticles on the bottom of culture dish by MAPLE could provide more possibilities for cell culture.