Reconstruction of Lower Jaw by Iliac Bone Graft, Experimental Study on Rabbit and Role of Mesenchymal Stem Cells

Abstract

page no: 20/24 www.ommegaonline.org Vol:4 Issue: 1 Abstract: In this research, twelve young male Rabbits age of 3 months are weighted 1.25 kg, divided in two groups of 5 Rabbits and 2 Rabbits were used as control. They were subjected to surgical osteoctomy by excising bone 1.5cm from the body of the mandible and bone graft of 2cm harvested from the iliac crest. The graft was fixed by soft stainless steel wire of 0.25 mm, post-operative systemic antibiotic of ceftriaxone of 20 mg / kg IM of body weight for six days prescribed, oxidized regenerated cellulose mesh was used to cover the graft of group B. After 3 months the experiment was terminated, the result were 2 Rabbits passed, one Rabbit end with infection and one with slight injury to Sciatic nerve without effect on mastication. The dyed Rabbits and one with infection were excluded from this experiment. The cytological changes of bone grafting showed formation of healthy granulation tissue with mesenchymal stem cells derived from bone marrow of bony segments of the mandible with platelets growth factor (PDGF) and from periosteum and covering muscles with large amount of fibroblasts with tiny blood vessels. Osteoblast was noticed more with chondrocyte and osteoid tissue with bone graft that covered by Surgical mesh (oxidized regenerated cellulose) soaked in blood and covered bone graft of group B to get faster healing in group B than in group A. The aim of this research to understand the cytological changes that occurred in the graft and recipient area.