Overview on capillary electrophoresis with mass spectrometry: Application in peptide analysis and proteomics

Abstract

The pharmaceutical industry and most particularly the biopharmaceutical industry are demanding advancements in analytical techniques. The field of proteomics and peptide mapping needs sensitive analysis and sophisticated instrumentation, which needs hyphenated analytical techniques to be explored. In addition to effective detection and characterization, Due to efficient and selective separation, predominant focus has been drawn to capillary electrophoresis/mass spectrometry (CE/MS) compared to widely used techniques such as slab-gel electrophoresis (SGE), liquid chromatography (LC) and capillary electrophoresis (CE) Several significant advances in CE/MS instrumentation and software have made CE/MS a competitive tool in the last decade to solve a wide variety of analytical and biopharmaceutical problems. This analysis discusses the technological aspects of CE / MS interfaces, analytical separation modes, MS analyzer, recent CE / MS approaches and applications specific to biopharmaceutical. Applications using some of the more common electrophoretic separation modes, such as conventional electrophoresis of the capillary zone (CZE) and capillary isoelectric focusing (CIEF), have been highlighted. The peptide analysis also includes recent studies on capillary electro-chromatography (CEC) / MS and CE-MS applications. Keywords: Capillary electrophoresis, Mass spectrometry, Pharmaceutical applications, Peptide analysis, Proteomics, Intact protein.