Isolation and Characterization of Anticancer Compound Producing Marine Paenibacillus lentimorbus SAGM 3 Collected from a Sea Anemone, Heteractis species

Nagamuthu Vinothkumar, P. Pugalendhi
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Abstract

Sea anemone-associated bacteria were considered promising candidates for the synthesis of many novel bioactive compounds. Thus culturable symbiotic bacteria that exist in the sea anemones met much attention when compared to other benthic marine dwellers. In this study, an attempt was made to explore the anticancer potentials of symbiotic bacteria isolated from a sea anemone, Heteractis species. Nine symbiotic bacteria were isolated, pure cultured and screened for their anticancer potential using two breast cancer cell lines. Among the strains, SAGM 3 showed appreciable growth inhibition activity of 43.1% and 47.1% against the studied cell lines, viz. MCF7 and MDA-MB-231 and this strain was selected for further studies. Based on the 16S rRNA molecular profiling, the SAGM 3 isolate was noted as Paenibacillus lentimorbus and the sequence of SAGM 3 was deposited in GeneBank with the accession number MW737456.1. During the growth kinetics profiling, maximum bacterial growth rate and anticancer activities were recorded from 60 to 96 hrs of incubation. The present investigation provides baseline data understanding the pharmaceutical significance of a symbiotic marine bacterium procured from the sea anemone, Heteractis species.
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海洋慢芽孢杆菌SAGM - 3抗癌化合物的分离与鉴定
海葵相关细菌被认为是合成许多新型生物活性化合物的有希望的候选者。因此,与其他底栖海洋生物相比,存在于海葵中的可培养共生细菌受到了更多的关注。本研究旨在探讨从海葵中分离的共生菌的抗癌潜力。利用两株乳腺癌细胞系分离、纯培养和筛选9种共生菌的抗癌潜力。其中,SAGM 3对MCF7和MDA-MB-231细胞株的生长抑制活性分别为43.1%和47.1%,选择该菌株进行进一步研究。根据16S rRNA分子图谱,鉴定该分离株为慢芽孢杆菌,并将其序列存入GeneBank,登录号为MW737456.1。在生长动力学分析过程中,从培养60到96小时记录了最大细菌生长速率和抗癌活性。本研究为了解从海葵(Heteractis)中提取的一种共生海洋细菌的药物意义提供了基础数据。
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