{"title":"Die Herstellung von Reptilien-Zellkulturen","authors":"W. Ahne","doi":"10.1111/J.1439-0450.1984.TB01344.X","DOIUrl":null,"url":null,"abstract":"Zusammenfassung \n \nDie mit einer Saline-Trypsin-Versenlosung dispergierten, von 3 Reptilienarten (Agamo stellio, Boa constrictor, Natrix natrix helvetica) abstammenden Herzzellen, liesen sich mit gutem Anzuchtserfolg mit MEM in vitro bei 25–30°C kultivieren. Nur die Gonaden der Boa constrictor erbrachten ahnliche Ergebnisse. Es konnten maximal 11 Subkulturen angelegt werden. \n \n \n \nSummary \n \nPreparation of tissue cultures from reptiles \n \n \n \nHeart cells of 3 reptiles species (Agamo stellio, Boa constrictor, Natrix natrix helvetica) obtained by a treatment with a saline trypsin versene solution grew well in MEM at 25–30°C. Gonads of the Boa constrictor could be cultivated with similar results only. The maximal subculture rate was 11. \n \n \n \nResumen \n \nLa elaboracion de cultivos celulares reptilianos \n \n \n \nCelulas cardiacas procedentes de 3 especies de reptiles (Agamo stellis, Boa constrictor, Natrix natrix helvetica), dispersadas con una solucion salina de tripsina y versenico, se pudieron cultivar in vitro con MEM a 25–30°C. Solo las gonadas de la Boa constrictor produjeron resultados similares. La tasa maxima de subcultivos era de 11.","PeriodicalId":17577,"journal":{"name":"Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health","volume":"30 6 1","pages":"634-636"},"PeriodicalIF":0.0000,"publicationDate":"2010-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/J.1439-0450.1984.TB01344.X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Zusammenfassung
Die mit einer Saline-Trypsin-Versenlosung dispergierten, von 3 Reptilienarten (Agamo stellio, Boa constrictor, Natrix natrix helvetica) abstammenden Herzzellen, liesen sich mit gutem Anzuchtserfolg mit MEM in vitro bei 25–30°C kultivieren. Nur die Gonaden der Boa constrictor erbrachten ahnliche Ergebnisse. Es konnten maximal 11 Subkulturen angelegt werden.
Summary
Preparation of tissue cultures from reptiles
Heart cells of 3 reptiles species (Agamo stellio, Boa constrictor, Natrix natrix helvetica) obtained by a treatment with a saline trypsin versene solution grew well in MEM at 25–30°C. Gonads of the Boa constrictor could be cultivated with similar results only. The maximal subculture rate was 11.
Resumen
La elaboracion de cultivos celulares reptilianos
Celulas cardiacas procedentes de 3 especies de reptiles (Agamo stellis, Boa constrictor, Natrix natrix helvetica), dispersadas con una solucion salina de tripsina y versenico, se pudieron cultivar in vitro con MEM a 25–30°C. Solo las gonadas de la Boa constrictor produjeron resultados similares. La tasa maxima de subcultivos era de 11.