Sarah E. Greene , Yuefang Huang , Wooseob Kim , Mariel J. Liebeskind , Vinay Chandrasekaran , Zhuoming Liu , Parakkal Deepak , Michael A. Paley , Daphne Lew , Monica Yang , Mehrdad Matloubian , Lianne S. Gensler , Mary C. Nakamura , Jane A. O'Hallaran , Rachel M. Presti , Sean P.J. Whelan , William J. Buchser , Alfred H.J. Kim , Gary J. Weil
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Abstract
Objective
Lateral flow assays (LFA) are sensitive for detecting antibodies to SARS-CoV-2 proteins within weeks after infection. This study tested samples from immunocompetent adults, and those receiving treatments for chronic inflammatory diseases (CID), before and after mRNA SARS-CoV-2 vaccination.
Methods
We compared results obtained with the COVIBLOCK Covid-19 LFA to those obtained by anti-spike (S) ELISA.
Results
The LFA detected anti-S antibodies in 29 of 29 (100%) of the immunocompetent and 110 of 126 (87.3%) of the CID participants after vaccination. Semiquantitative LFA scores were statistically significantly lower in samples from immunosuppressed participants, and were significantly correlated with anti-S antibody levels measured by ELISA.
Conclusions
This simple LFA test is a practical alternative to laboratory-based assays for detecting anti-S antibodies after infection or vaccination. This type of test may be most useful for testing people in outpatient or resource-limited settings.
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